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A propos de cet article
UNSPSC Code:
41105600
NACRES:
NA.54
Form:
solution
Recombinant:
expressed in E. coli
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Laissez-nous vous aiderrecombinant
expressed in E. coli
form
solution
usage
sufficient for 20 tests
packaging
pkg of 2 × 50 μL
manufacturer/tradename
Roche
storage temp.
−20°C
Quality Level
Catégories apparentées
General description
TUNEL enzyme solution, containing terminal deoxynucleotidyl transferase from calf thymus recombinant in E. coli.
One of the two components required for performing the TUNEL reaction to detect apoptosis in situ.
One of the two components required for performing the TUNEL reaction to detect apoptosis in situ.
Application
The enzyme solution contains terminal deoxynucleotidyl transferase from calf thymus, recombinant in E. coli, needed to perform the TUNEL reaction for detecting apoptosis in situ.
The TUNEL reaction mixture is used to label DNA strand breaks for detecting and quantitating apoptotic cell death at the single-cell level in cells and tissues.
The TUNEL reaction mixture is used to label DNA strand breaks for detecting and quantitating apoptotic cell death at the single-cell level in cells and tissues.
Physical form
Terminal deoxynucleotidyl transferase (TdT) in an optimized concentration, dissolved in reaction buffer. Ready-to-use with TUNEL Label.
Preparation Note
Working solution: TUNEL Enzyme is used in combination with TUNEL Label to prepare the TUNEL reaction mixture.
To produce a TUNEL solution sufficient for one test:
Storage conditions (working solution): Note: The TUNEL reaction mixture (45μl TUNEL Label with 5μl TUNEL Enzyme for 1 test) should be prepared immediately before use and should not be stored. Keep TUNEL reaction mixture on ice until use.
To produce a TUNEL solution sufficient for one test:
- Mix 5μl TUNEL Enzyme with 45μl TUNEL Label prior to use.
- For negative control use 50μl/test TUNEL Label only.
Storage conditions (working solution): Note: The TUNEL reaction mixture (45μl TUNEL Label with 5μl TUNEL Enzyme for 1 test) should be prepared immediately before use and should not be stored. Keep TUNEL reaction mixture on ice until use.
Other Notes
For life science research only. Not for use in diagnostic procedures.
Classe de stockage
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
does not flash
flash_point_c
does not flash
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