Se connecter pour consulter les tarifs organisationnels et contractuels.
Sélectionner une taille de conditionnement
Changer de vue
A propos de cet article
UNSPSC Code:
12352203
NACRES:
NA.54
Conjugate:
peroxidase conjugate
Clone:
clone 3F10, monoclonal
Application:
—
Citations:
377
Service technique
Besoin d'aide ? Notre équipe de scientifiques expérimentés est là pour vous.
Laissez-nous vous aiderbiological source
rat
conjugate
peroxidase conjugate
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
clone 3F10, monoclonal
form
lyophilized (clear, colorless solution after reconstitution)
packaging
pkg of 25 U (25 μg)
manufacturer/tradename
Roche
isotype
IgG1
epitope sequence
YPYDVPDYA
storage temp.
2-8°C
General description
Anti-HA-Peroxidase, High Affinity is a monoclonal antibody to the HA-peptide (clone 3F10), conjugated to peroxidase.
The Anti-HA High Affinity antibody (clone 3F10) recognizes the same epitope as clone 12CA5, which was originally used to study how the immune system recognizes the influenza hemagglutinin protein, a surface glycoprotein required for infectivity of the human virus. However, the principal use of the Anti-HA antibody is the detection and purification of proteins whose encoding DNA sequences have been fused to the HA epitope sequence by recombinant techniques, that is epitope tagging. The ability to prepare such epitope-tagged proteins and locate them with the Anti-HA antibody in subsequent experiments has enabled researchers to determine:
- The size, cellular localization, and abundance of proteins produced by newly discovered genes.
- Post-translational modifications of proteins.
- The movement of proteins within cell membranes.
- The identity of proteins within functional protein complexes.
- The function of proteins that are unstable, difficult to purify, or share epitopes with a number of other proteins.
Immunogen
The epitope consists of amino acids 98-106 from the human influenza virus hemagglutinin protein.
Application
- Use Anti-HA-Peroxidase, High Affinity for the detection of HA-tagged recombinant proteins using: ELISA
- Western blot
Biochem/physiol Actions
Anti-HA-Peroxidase, High Affinity (3F10) recognizes the 9-amino acid sequence YPYDVPDYA, derived from the human influenza hemagglutinin (HA) protein.
This epitope is also recognized in fusion proteins regardless of its position (N-terminal, C-terminal or internal).
This epitope is also recognized in fusion proteins regardless of its position (N-terminal, C-terminal or internal).
Preparation Note
Stabilizers: proteinaceous stabilizers
Working concentration: The working concentration of conjugate depends on application and substrate.
The following concentrations should be taken as a guideline:
Working concentration: The working concentration of conjugate depends on application and substrate.
The following concentrations should be taken as a guideline:
- Dot blot: 50 mU/ml
- ELISA: 25 mU/ml
- Western blot: 50 mU/ml
Add 1.0 ml double-distilled water for a final concentration of 25 U/mL.
Rehydrate for 10 min prior to use.
Rehydrate for 10 min prior to use.
Other Notes
For life science research only. Not for use in diagnostic procedures.
Still not finding the right product?
Essayez notre Outil de sélection de produits pour affiner vos choix.
signalword
Warning
hcodes
Hazard Classifications
Skin Sens. 1
Classe de stockage
11 - Combustible Solids
wgk
WGK 1
flash_point_f
does not flash
flash_point_c
does not flash
Faites votre choix parmi les versions les plus récentes :
Déjà en possession de ce produit ?
Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Contenu apparenté
Regina Gratz et al.
The New phytologist, 225(1), 250-267 (2019-09-06)
The key basic helix-loop-helix (bHLH) transcription factor in iron (Fe) uptake, FER-LIKE IRON DEFICIENCY-INDUCED TRANSCRIPTION FACTOR (FIT), is controlled by multiple signaling pathways, important to adjust Fe acquisition to growth and environmental constraints. FIT protein exists in active and inactive
Ranran Wang et al.
Cancers, 12(6) (2020-06-25)
The epigenetic reader BRD4 binds acetylated histones and plays a central role in controlling cellular gene transcription and proliferation. Dysregulation of BRD4's activity has been implicated in the pathogenesis of a wide variety of cancers. While blocking BRD4 interaction with
Jihoon Ha et al.
International journal of molecular sciences, 21(9) (2020-05-02)
p62/sequestosome-1 is a scaffolding protein involved in diverse cellular processes such as autophagy, oxidative stress, cell survival and death. It has been identified to interact with atypical protein kinase Cs (aPKCs), linking these kinases to NF-κB activation by tumor necrosis