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Merck

01696

Acrylamide

for Northern and Southern blotting, powder blend

Synonyme(s) :

2-Propenamide, Polyacrylamide, acrylic amide, akrylamid, Acrylic acid amide

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A propos de cet article

Formule linéaire :
CH2=CHCONH2
Numéro CAS:
Poids moléculaire :
71.08
NACRES:
NA.25
PubChem Substance ID:
eCl@ss:
39031205
UNSPSC Code:
41105319
EC Number:
201-173-7
MDL number:
Beilstein/REAXYS Number:
605349
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grade

Molecular Biology

Quality Level

vapor density

2.45 (vs air)

vapor pressure

0.03 mmHg ( 40 °C)

product line

BioReagent

assay

≥99.5% (GC)

form

powder blend

technique(s)

electrophoresis: suitable

impurities

DNases, none detected, RNases, none detected, phosphatases, none detected, proteases, none detected

bp

125 °C/25 mmHg (lit.)

mp

82-86 °C (lit.)

solubility

H2O: 0.25 g/mL at 20 °C, clear, colorless

storage temp.

2-8°C

SMILES string

NC(=O)C=C

InChI

1S/C3H5NO/c1-2-3(4)5/h2H,1H2,(H2,4,5)

InChI key

HRPVXLWXLXDGHG-UHFFFAOYSA-N

General description

Acrylamide powder is a white, odorless, crystalline solid. It is commonly used in molecular biology labs as a cross linking agent to cast polyacrylamide gels for nucleic acid electrophoresis.

Application

Suitable for making polyacrylamide gels according to established recipes.

Disclaimer

Acrylamide powder is highly toxic through inhalation and skin contact. Acrylamide is considered to be a probable carciongen in humans. Standard laboratory nitrile gloves and full sleeve lab coats should be worn when handling acrylamide in any form. Dust masks or working in a fume hood is advised when handling acrylamide powder.


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pictograms

Skull and crossbonesHealth hazard

signalword

Danger

Hazard Classifications

Acute Tox. 3 Oral - Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Carc. 1B - Eye Irrit. 2 - Muta. 1B - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 1 Oral

target_organs

Peripheral nervous system

Classe de stockage

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk

WGK 3

flash_point_f

280.4 °F - closed cup

flash_point_c

138 °C - closed cup

ppe

Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges



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R.C. Ogden et al.
Methods in Enzymology, 152, 66-66 (1987)
Arianna Rath et al.
Proceedings of the National Academy of Sciences of the United States of America, 110(39), 15668-15673 (2013-09-11)
SDS/PAGE is universally used in biochemistry, cell biology, and immunology to resolve minute protein amounts readily from tissue and cell extracts. Although molecular weights of water-soluble proteins are reliably determined from their SDS/PAGE mobility, most helical membrane proteins, which comprise
Jonas S Laursen et al.
Journal of the American Chemical Society, 135(7), 2835-2844 (2013-01-25)
Non-natural peptide analogs have significant potential for the development of new materials and pharmacologically active ligands. One such architecture, the β-peptoids (N-alkyl-β-alanines), has found use in a variety of biologically active compounds but has been sparsely studied with respect to