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Merck

114413

Bromothymol Blue

ACS reagent, Dye content 95 %

Synonyme(s) :

3′,3″-Dibromothymolsulfonphthalein

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A propos de cet article

Formule empirique (notation de Hill) :
C27H28Br2O5S
Numéro CAS:
76-59-5
Poids moléculaire :
624.38
UNSPSC Code:
12171500
NACRES:
NA.47
PubChem Substance ID:
24847171
EC Number:
200-971-2
Beilstein/REAXYS Number:
373934
MDL number:
MFCD00005872

Principaux documents

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InChI

1S/C27H28Br2O5S/c1-13(2)17-11-20(15(5)23(28)25(17)30)27(19-9-7-8-10-22(19)35(32,33)34-27)21-12-18(14(3)4)26(31)24(29)16(21)6/h7-14,30-31H,1-6H3

InChI key

NUHCTOLBWMJMLX-UHFFFAOYSA-N

SMILES string

CC(C)c1cc(c(C)c(Br)c1O)C2(OS(=O)(=O)c3ccccc23)c4cc(C(C)C)c(O)c(Br)c4C

grade

ACS reagent

form

powder

composition

Dye content, 95%

color

pink-purple

visual transition interval

6.0-7.6, yellow to blue(passes test)

mp

200-202 °C ((392 - 396 °F) - lit.), 200-202 °C (lit.)

λmax

420 nm

ε (extinction coefficient)

≥17000 at 419-425 nm in methanol, ≥8000 at 326-332 nm in methanol, ≥9000 at 279-285 nm in methanol

storage temp.

room temp

Quality Level

200

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Catégories apparentées

General description

Bromothymol Blue, also known as, dibromothymolsulfonphthalein is a sulfonated hydroxyquinone in acidic aqueous solutions. In alkaline solutions, this dye is present as a moderately sized dianion. It is also known as bromthymol blue and dibromothymolsulfonphthalein. It is a pH-sensitive dye that changes color from yellow to blue.

Application

Bromothymol blue dye serves as a pH indicator because of the precise color transition at neutrality. Other applications include:
  • as a vital stain to trace the movement of fluids from the lymph and to define cell walls or nuclei under the microscope
  • for the demonstration of fungal hyphae within plant roots
  • in the assessment of the soluble acidity within single rice grains
  • use in an agar gel medium for enumeration of Bacillus cereus
  • as a component of several bacterial growth and detection substrates such as cystine lactose electrolyte deficient (CLED/BROLACIN); polymyxin pyruvate egg yolk mannitol bromothymol blue (PEMBA) and MacConkey agar.

Classe de stockage

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificats d'analyse (COA)

Lot/Batch Number
0000352454
0000352443
0000352454
0000352443
0000293123

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Reza Amin et al.
Micromachines, 11(6) (2020-07-01)
To transform from reactive to proactive healthcare, there is an increasing need for low-cost and portable assays to continuously perform health measurements. The paper-based analytical devices could be a potential fit for this need. To miniaturize the multiplex paper-based microfluidic
Andrea Pastore et al.
Talanta, 219, 121251-121251 (2020-09-06)
A pH colorimetric sensor array (CSA) with fast response time (<1 min) using only one acid-base indicator, Bromothymol Blue (BB), was prepared and characterized by modulating the amount, C, of the surfactant Hexadecyltrimethylammonium p-toluenesulfonate between 0 and 0.3725 gCTApTs/gprecursor with a
Hisataka Maruyama et al.
Lab on a chip, 8(2), 346-351 (2008-01-31)
This paper demonstrates local pH measurement in a microchip using a pH-sensing gel-microbead. To achieve this, the gel-microbead made of a hydrophilic photo-crosslinkable resin was functionalized with the pH indicator bromothymol blue (BTB). The primary constituent of this photo-crosslinkable resin
Olga A Zaporozhets et al.
Analytica chimica acta, 597(1), 171-177 (2007-07-31)
Solid-phase spectrophotometric and visual test-methods of fluoride and oxalate determination are proposed. The methods are based on the competitive reactions of ZrOCl2 with methylthymol blue immobilized on silica gel and fluoride or oxalate in solution. Absorbance of the solid-phase reagent
Kristina Jurcic et al.
Journal of chromatography. A, 1134(1-2), 317-325 (2006-10-07)
An effective sample preconcentration technique for proteins and peptides was recently developed using capillary electrophoresis (CE) with discontinuous buffers [C.A. Nesbitt, J.T.-M. Lo, K.K.-C. Yeung, J. Chromatogr. A 1073 (2005) 175]. Two buffers of different pH created a junction to
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