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Merck

48900

Giemsa Stain

Modified Solution

Synonyme(s) :

Azure eosin methylene blue, Giemsa solution

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A propos de cet article

Numéro CAS:
51811-82-6
UNSPSC Code:
41116121
PubChem Substance ID:
329757421
NACRES:
NA.25
EC Number:
200-659-6
MDL number:
MFCD00081642

Principaux documents

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Nom du produit

Giemsa Stain, Modified Solution, (for the staining (of cellular blood components and blood parasites))

SMILES string

[Cl-].CN(C)c1ccc2N=C3C=CC(=[NH2+])C=C3Sc2c1

InChI key

NALREUIWICQLPS-UHFFFAOYSA-N

InChI

1S/C14H13N3S.ClH/c1-17(2)10-4-6-12-14(8-10)18-13-7-9(15)3-5-11(13)16-12;/h3-8,15H,1-2H3;1H

type

(for research use only)

shelf life

limited shelf life

technique(s)

microbe id | staining: suitable

suitability

Chlamydia spp.
Histoplasma spp.
Plasmodium spp.
Trichomonas spp.
bacteria
protozoa
spirochetes

Quality Level

200

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Catégories apparentées

Application

Tested for staining blood smears according to G. Clark (ed.), Staining Procedures, 4th edition, Williams & Wilkins, 173 (1981); together with May-Grünwald Solution for pap staining according to Pappenheim.

Biochem/physiol Actions

When blood films are stained using Giemsa Stain, the nucleus and cytoplasm of white blood cells take on characteristic blue or pink coloration. The use of purified eosin and thiazine dyes minimizes lot-to-lot variation.

Disclaimer

store at 20°C

Physical form

Solution of azure B/azure II-eosin/methylene blue 1:12:2 (w/w/w) in glycerol/methanol 5:24 (v/v); total dye content: 0.6 % (w/w)

Preparation Note

Fix 10 min. in methanol; air-dry; immerse 45 min. in Giemsa Solution; rinse with distilled water; air-dry

signalword

Danger

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Flam. Liq. 2 - STOT SE 1

target_organs

Eyes

Classe de stockage

3 - Flammable liquids

wgk

WGK 2

flash_point_f

53.6 °F

flash_point_c

12 °C

ppe

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

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Certificats d'analyse (COA)

Lot/Batch Number
BCCN5130
BCCM1795
BCCJ6418
BCCH4877
BCCG6252

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Alessandra Micera et al.
PloS one, 10(11), e0142737-e0142737 (2015-11-17)
In a previous study, we reported the upregulation of Nerve Growth Factor (NGF) and trkANGFR expression in Ocular Cicatricial Pemphigoid (OCP), an inflammatory and remodeling eye disease. Herein, we hypothesize a potential NGF-driven mechanism on fibroblasts (FBs) during OCP remodeling
Jenő Káldy et al.
Genes, 11(7) (2020-07-10)
Two species from the families Acipenseridae and Polyodontidae, Russian sturgeon (Acipenser gueldenstaedtii, Brandt and Ratzeberg, 1833; functional tetraploid) and American paddlefish (Polyodon spathula, Walbaum 1792, functional diploid) were hybridized. The hybridization was repeated using eggs from three sturgeon and sperm
Tobias Koeniger et al.
Stem cells (Dayton, Ohio), 39(2), 227-239 (2020-12-04)
Although the bone marrow contains most hematopoietic activity during adulthood, hematopoietic stem and progenitor cells can be recovered from various extramedullary sites. Cells with hematopoietic progenitor properties have even been reported in the adult brain under steady-state conditions, but their
Antonio Di Zazzo et al.
Journal of molecular medicine (Berlin, Germany), 98(5), 751-760 (2020-04-22)
Metabolomics has been applied to diagnose diseases, predict disease progression, and design therapeutic strategies in various areas of medicine. However, it remains to be applied to the ocular surface diseases, where biological samples are often of limited quantities. We successfully
Liz J Valente et al.
The Journal of cell biology, 219(11) (2020-09-05)
The mechanisms by which TP53, the most frequently mutated gene in human cancer, suppresses tumorigenesis remain unclear. p53 modulates various cellular processes, such as apoptosis and proliferation, which has led to distinct cellular mechanisms being proposed for p53-mediated tumor suppression
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Contenu apparenté

Data Sheet - 48900

48900 Giemsa Stain, Modified Solution

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