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67309

Kovac′s reagent for indoles

suitable for microbiology

Synonyme(s) :

4-(Dimethylamino)benzaldehyde solution

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A propos de cet article

Formule empirique (notation de Hill) :
C9H11NO
Numéro CAS:
100-10-7
Poids moléculaire :
149.19
UNSPSC Code:
41171621
NACRES:
NA.85
PubChem Substance ID:
329760541
MDL number:
MFCD00003381
Beilstein/REAXYS Number:
4132845
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agency

according to ISO 16654:2001

Quality Level

200

product line

BioChemika

shelf life

limited shelf life, expiry date on the label

composition

4-(dimethlyamino)benzaldehyde, 50 g/L , hydrochloric acid, 240 g/L , isoamylic alcohol, 710 g/L

technique(s)

microbe id | specific enzyme detection: suitable

application(s)

agriculture
clinical testing
environmental
food and beverages, microbiology

storage temp.

2-8°C

suitability

Escherichia coli, coliforms

SMILES string

[H]C(=O)c1ccc(cc1)N(C)C

InChI

1S/C9H11NO/c1-10(2)9-5-3-8(7-11)4-6-9/h3-7H,1-2H3

InChI key

BGNGWHSBYQYVRX-UHFFFAOYSA-N

General description

Kovac′s reagent is prepared by mixing p-dimethylaminobenzaldehyde, isoamyl alcohol and concentrated hydrochloric acid. For identification of an organism, the formation of Indole from a tryptophan substrate is a useful diagnostic tool. Indole production is a crucial test in identification of Escherichia coli. After incubation, adding the reagent will help in determination if Indole that has been produced. The reagent turns red when reacts with Indole.

Application

In the presence of oxygen, some bacteria, like E.coli, are able to split tryptophan into indole and alpha-aminopropionic acid. This reagent is for detecting the indole and identify the indole-positive and indole-negative microorganisms.
Kovac′s reagent may be used for spot test for determination of indole by saturating filter paper with the reagent. It may also be used for initial spot test for determination of indole followed by quantification by spectrophotometric assay and HPLC-UV/Vis-MS/MS methods.

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Danger

Hazard Classifications

Acute Tox. 4 Inhalation - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Skin Corr. 1B - Skin Sens. 1 - STOT SE 3

target_organs

Respiratory system

Classe de stockage

3 - Flammable liquids

wgk

WGK 3

flash_point_f

109.4 °F

flash_point_c

43 °C

ppe

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter

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Certificats d'analyse (COA)

Lot/Batch Number
BCCN2957
BCCL5138
BCCK4337
BCCH8824
BCCG3914

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Contenu apparenté

Rita R. Colwell, R. Grigorova
Methods in Microbiology, 19, 27-27 (1987)
S. Harisha
An Introduction to Practical Biotechnology, 193-193 (2005)
Patrick R Porubsky et al.
Archives of biochemistry and biophysics, 475(1), 14-17 (2008-04-22)
Cytochrome P450 2A13 (CYP2A13) is a lung specific enzyme known to activate the potent tobacco procarcinogen 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) into two carcinogenic metabolites. CYP2A13 has been crystallized and X-ray diffraction experiments illuminated the structure of this enzyme, but with an unknown
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