Skim Milk Powder for Biotechnology and Microbiology

Skim Milk Powder is recommended for cultivation and enumeration of microorganisms encountered in dairy industry

Skim Milk powder is used for the demonstration of coagulation and proteolysis of casein. Skim Milk is sometimes used as a complete medium or as an ingredient in other media used for the propagation of organisms occurring in milk products. The addition of skim milk to any nutrient-rich medium creates favorable conditions for the growth of organisms, which are encountered in milk. The number of bacteria isolated thus is more than the number of organisms isolated on a regular medium. Proteolytic bacteria hydrolyze casein to form soluble nitrogenous compounds indicated as a clear zone surrounding the colonies on the agar medium. Skim milk serves as a good source of casein

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Mix the powder to a smooth paste with a small quantity of distilled water, then gradually add more distilled water until a 10% w/v mixture is obtained. This is equivalent to fresh milk, and may be sterilized by autoclaving for 5 minutes at 121 °C. Care should be taken not to overheat during sterilization, otherwise caramelization will occur.