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Merck

90903

Schaeffer and Fulton Spore Stain Solution A

suitable for microscopy

Synonyme(s) :

Malachite Green Solution according to Schaeffer and Fulton

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A propos de cet article

Numéro CAS:
2437-29-8
UNSPSC Code:
12171500
NACRES:
MA.02
MDL number:
MFCD06412418

Principaux documents

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SMILES string

[N+](=C4C=CC(=C(c6ccc(cc6)N(C)C)c5ccccc5)C=C4)(C)C.[N+](=C1C=CC(=C(c3ccc(cc3)N(C)C)c2ccccc2)C=C1)(C)C.[O-]C(=O)C(=O)[O-].OC(=O)C(=O)O.OC(=O)C(=O)O

InChI

1S/2C23H25N2.3C2H2O4/c2*1-24(2)21-14-10-19(11-15-21)23(18-8-6-5-7-9-18)20-12-16-22(17-13-20)25(3)4;3*3-1(4)2(5)6/h2*5-17H,1-4H3;3*(H,3,4)(H,5,6)/q2*+1;;;/p-2

InChI key

CNYGFPPAGUCRIC-UHFFFAOYSA-L

product line

BioChemika

form

solution

shelf life

limited shelf life, expiry date on the label

technique(s)

microbe id | staining: suitable

suitability

suitable for microscopy, spore forming microorganisms

General description

In Schaeffer-Fulton`s method, a primary stain-malachite green is forced into the spore by steaming the bacterial emulsion. Malachite green is water soluble and has a low affinity for cellular material, so vegetative cells may be decolorized with water. Vegetative cells are then counterstained with safarin (Schaeffer & Fulton`s Spore Stain B, Cat. No. 39955). The components and the method are also available as kit (Cat. No. 04551).

Other Notes

Composition:
Schaeffer & Fulton`s Spore Stain A: Malachite green 50 g/l in water

signalword

Danger

Hazard Classifications

Aquatic Chronic 2 - Eye Dam. 1 - Repr. 2

Classe de stockage

12 - Non Combustible Liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

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Certificats d'analyse (COA)

Lot/Batch Number
BCCN4849
BCCM7613
BCCK0823
BCCK9351
BCCJ6790

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Jia Zeng et al.
Biophysical journal, 104(1), 139-145 (2013-01-22)
It is shown that the nonlinear optical phenomenon known as second-harmonic generation can be used for label-free, time-resolved study of the transport of molecules through living cell membranes. The adsorption and transport of a 300-Da molecular-mass hydrophobic ion at the
Xiaofang Jia et al.
Chemistry (Weinheim an der Bergstrasse, Germany), 18(42), 13494-13500 (2012-09-05)
This work describes a novel strategy for the highly sensitive and selective detection of cysteine (Cys) and glutathione (GSH) based on the Hg(2+)-AGRO100-malachite green (MG) complex system. The dye MG, which has a very low quantum yield in aqueous solution
Yang Wu et al.
Cytometry. Part A : the journal of the International Society for Analytical Cytology, 83(2), 220-226 (2013-01-11)
Monitoring the trafficking of multiple proteins simultaneously in live cells is of great interest because many receptor proteins are found to function together with others in the same cell. However, existing fluorescent labeling techniques have restricted the mechanistic study of
R Sahraei et al.
Environmental monitoring and assessment, 185(7), 5817-5822 (2012-12-04)
A new spectrophotometric method is reported for the determination of nanomolar level of malachite green in surface water samples. The method is based on the catalytic effect of silver nanoparticles on the oxidation of malachite green by hexacyanoferrate (III) in
Ola M Gomaa et al.
Cell biochemistry and biophysics, 65(3), 413-423 (2012-10-19)
Filamentous fungi show great promise in remediation of environmental contaminants such as industrial dyes. In the current study, Aspergillus niger (Genbank ID: JF437542) decolorized 82 % of the test dye malachite green (MG; 50 mg/l) during cultivation for 24 h.
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