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Merck

93595

Bleu de trypan solution

0.4%, suitable for microscopy

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A propos de cet article

Formule empirique (notation de Hill) :
C34H24N6Na4O14S4
Numéro CAS:
72-57-1
Poids moléculaire :
960.81
UNSPSC Code:
12171500
NACRES:
MA.02
PubChem Substance ID:
329770254
MDL number:
MFCD00003969
Beilstein/REAXYS Number:
4360496

Principaux documents

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Nom du produit

Bleu de trypan solution, 0.4%, suitable for microscopy

InChI

1S/C34H28N6O14S4.4Na/c1-15-7-17(3-5-25(15)37-39-31-27(57(49,50)51)11-19-9-21(55(43,44)45)13-23(35)29(19)33(31)41)18-4-6-26(16(2)8-18)38-40-32-28(58(52,53)54)12-20-10-22(56(46,47)48)14-24(36)30(20)34(32)42;;;;/h3-14,41-42H,35-36H2,1-2H3,(H,43,44,45)(H,46,47,48)(H,49,50,51)(H,52,53,54);;;;/q;4*+1/p-4

SMILES string

[Na+].[Na+].[Na+].[Na+].Cc1cc(ccc1N=Nc2c(O)c3c(N)cc(cc3cc2S([O-])(=O)=O)S([O-])(=O)=O)-c4ccc(N=Nc5c(O)c6c(N)cc(cc6cc5S([O-])(=O)=O)S([O-])(=O)=O)c(C)c4

InChI key

GLNADSQYFUSGOU-UHFFFAOYSA-J

form

liquid

shelf life

limited shelf life, expiry date on the label

concentration

0.4%

technique(s)

microbe id | staining: suitable

color

blue to very dark blue

density

1.007 g/mL at 20 °C

εmax

1.4 at 603 nm in methanol

suitability

suitable for microscopy

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

Quality Level

200

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Catégories apparentées

Application

Trypan blue applications include:
  • it is a routinely used for viability testing for diverse cell types including frozen sperm and aortic muscle cells exposed to antifungal agents.
  • employed as a collagen stain in a Van Gieson procedure
  • To stain amyloid, Trypan blue is considered as a blue alternative to Congo red in Puchtler-Bennhold stains.
  • used as a fluorescent tracer of cell populations in embryology
  • used as a tumor promoter modulating permeability of lysosomal membranes
Trypan Blue solution has also been used in the cytotoxity tests and to determine the vital cells for the analysis of cell division.

Biochem/physiol Actions

Trypan Blue is excluded by most living cells, but can be taken into phagocytes and certain other cells.

General description

Trypan Blue is a tetrasulfonated anionic, hydrophilic azo dye derived from toluidine. The non-permeable cell membrane dye is a vital dye that selectively stains dead or non-viable cells, that pass through damaged but not through intact cell membranes.

Physical form

0.4% in 0.81% sodium chloride; 0.06% potassium phosphate, dibasic

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H350

Hazard Classifications

Carc. 1B

Classe de stockage

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type ABEK (EN14387) respirator filter

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Certificats d'analyse (COA)

Lot/Batch Number
BCCN6617
BCCN6618
BCCM6690
BCCM0751
BCCM5760

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Ulrich Putz et al.
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