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Merck

A8097

Acridine Orange hydrochloride solution

>95.0% purity (HPLC), solution

Synonyme(s) :

3,6-Bis(dimethylamino)acridine hydrochloride

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About This Item

Formule empirique (notation de Hill) :
C17H19N3 · HCl
Numéro CAS:
65-61-2
Poids moléculaire :
301.81
NACRES:
NA.47
PubChem Substance ID:
24891317
UNSPSC Code:
12171500
MDL number:
MFCD00012660
Beilstein/REAXYS Number:
3575587

Nom du produit

Acridine Orange hydrochloride solution, 10 mg/mL in H2O, ≥95.0% (HPLC)

InChI

1S/C17H19N3.ClH/c1-19(2)14-7-5-12-9-13-6-8-15(20(3)4)11-17(13)18-16(12)10-14;/h5-11H,1-4H3;1H

SMILES string

Cl[H].CN(C)c1ccc2cc3ccc(cc3nc2c1)N(C)C

InChI key

VSTHNGLPHBTRMB-UHFFFAOYSA-N

assay

≥95.0% (HPLC)

form

solution

concentration

10 mg/mL in H2O

technique(s)

microbe id | staining: suitable

color

orange to red

εmax

47-54 at 486-492 nm in water

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

Quality Level

200

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Catégories apparentées

Application

Acridine Orange hydrochloride solution has been used to study autophagic cell death. It has also been used for the staining of chromosomes.

Biochem/physiol Actions

Acridine Orange is a metachromatic dye which can stain DNA, RNA and acid glycosaminoglycans. At low concentration it intercalates into DNA and precipitates RNA. However, at high concentration it denatures and precipitates both RNA and DNA. Acridine Orange is also used to analyze autophagy. It goes into acidic organelles in a pH-dependent manner. At neutral pH, acridine orange gives a green fluorescence and in acidic conditions, it accumulates in the acidic organelle giving a bright red fluorescence.

pictograms

Health hazard
GHS08

signalword

Warning

hcodes

H341

Hazard Classifications

Muta. 2

Classe de stockage

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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Certificats d'analyse (COA)

Lot/Batch Number
0000331473
0000306280
0000306280
0000331473
0000285461

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Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology], 50(3), 791-805 (2019-06-30)
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Environmental science and pollution research international, 26(24), 24911-24921 (2019-06-28)
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