H3149
Héparine sodium salt from porcine intestinal mucosa
Grade I-A, ≥180 USP units/mg, powder, BioReagent, suitable for cell culture
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A propos de cet article
Nom du produit
Héparine sodium salt from porcine intestinal mucosa, Grade I-A, ≥180 USP units/mg, powder, BioReagent, suitable for cell culture
SMILES string
[Na+].[S](=O)(=O)(NC1C(OC(C(C1O[S](=O)(=O)O)OC4OC(C(C(C4O[S](=O)(=O)O)O)O)C(=O)O)CO)OC2C(OC(C(C2O)O[S](=O)(=O)O)OC3C(OC(C(C3O)NC(=O)C)O)CO[S](=O)(=O)O)C(=O)O)O
InChI
1S/C26H42N2O37S5.Na/c1-4(30)27-7-9(31)13(6(56-23(7)39)3-55-67(43,44)45)58-26-19(65-70(52,53)54)12(34)16(20(62-26)22(37)38)60-24-8(28-66(40,41)42)15(63-68(46,47)48)14(5(2-29)57-24)59-25-18(64-69(49,50)51)11(33)10(32)17(61-25)21(35)36;/h5-20,23-26,28-29,31-
InChI key
JRTRSJGZMRQDHI-UHFFFAOYSA-N
biological source
Porcine intestinal mucosa
type
Grade I-A
product line
BioReagent
form
powder
specific activity
≥180 USP units/mg
technique(s)
analytical sample preparation: suitable
cell culture | mammalian: suitable
color
beige
solubility
H2O: 50 mg/mL
application(s)
cell analysis
diagnostic assay manufacturing
life science and biopharma
sample preparation
Quality Level
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Application
- Le sel sodique d′héparine provenant de la muqueuse intestinale porcine a été utilisé pour la purification de sLRP (soluble circulating low density lipoprotein receptor-related protein-1) à partir de plasma humain.
- Elle a été utilisée comme supplément dans le milieu M199 pour la culture des cellules endothéliales de veine ombilicale humaine.
- Elle a servi d′agent anticoagulant lors de l′isolement de sang.
Features and Benefits
- Composé de grande pureté convenant à une grande variété d'applications de recherche.
General description
Other Notes
wgk
WGK 2
Classe de stockage
11 - Combustible Solids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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How is Heparin sodium salt from porcine intestinal mucosa, Product H3149, solubilized?
Heparin sodium salt from porcine intestinal mucosa is soluble in deionized water at a concentrationi of 50 mg/mL.
How much of Heparin sodium salt from porcine intestinal mucosa, Product H3149, is used for cell culture?
Solutions at 0.5 mg per mL were tested in cell culture. Start at this concentration, and titer from there to determine the optimal concentration for usage.
How are solutions Heparin sodium salt from porcine intestinal mucosa, Product H3149, stored?
Solutions can be stored at 2-8C for up to 2 years, if sterile filtered through a 0.22 micron filter. Benzyl alcohol (0.06-1%) can be added to solutions to prevent microbial growth.
How many milligrams of Heparin sodium salt, Product H3149, are in the bottle?
The potency, in units/mg, is lot-specific; the value can be found on the certificate of analysis. It is typically over 140 units per mg. The number of milligrams can be calculated by dividing the number of units by the potency in units per mg.
What is the molecular weight of Heparin sodium salt?
Heparin is a mixture of polyanion chains having molecular weights ranging from 6,000 to 30,000 Daltons, with most chains in the range of 17,000 to 19,000 Daltons.
How much Heparin sodium salt, is used to prevent coagulation?
We suggest using 20 to 50 units per mL of blood.
What is the best way to sterilize Heparin sodium salt?
Although solutions may be autoclaved at 121 °C for 5 to 10 minutes, we generally recommend sterile filtration. This is because prolonged autoclaving may have an effect on its activity.
What concentration of Heparin sodium salt, can I use to inhibit RNAse activity?
Heparin has been used at concentrations ranging from 100 to 500 μg/mL. This has been reported in the J. Biol. Chem., 248, 2095 (1973).
Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I find price and availability?
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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