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Merck

32211

Chloroforme

puriss. p.a., reag. ISO, reag. Ph. Eur., 99.0-99.4% (GC)

Synonyme(s) :

Trichlorométhane, Trichlorure de méthylidyne

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About This Item

Formule empirique (notation de Hill) :
CHCl3
Numéro CAS:
Poids moléculaire :
119.38
UNSPSC Code:
12191502
NACRES:
NA.04
PubChem Substance ID:
EC Number:
200-663-8
Beilstein/REAXYS Number:
1731042
MDL number:
assay:
99.0-99.4% (GC)
technique(s):
RNA extraction: suitable
bp:
60.5-61.5 °C (lit.)
vapor pressure:
160 mmHg ( 20 °C)

Nom du produit

Chloroforme, puriss. p.a., reag. ISO, reag. Ph. Eur., 99.0-99.4% (GC)

InChI key

HEDRZPFGACZZDS-UHFFFAOYSA-N

InChI

1S/CHCl3/c2-1(3)4/h1H

SMILES string

ClC(Cl)Cl

agency

USP/NF
reag. ISO
reag. Ph. Eur.

vapor density

4.1 (vs air)

vapor pressure

160 mmHg ( 20 °C)

grade

puriss. p.a.

assay

99.0-99.4% (GC)

form

liquid

contains

~1% ethanol as stabilizer

technique(s)

RNA extraction: suitable

impurities

≤0.00001% free chlorine (Cl)
≤0.00005% free acid (as HCl)
≤0.0005% non-volatile matter
≤0.005% aldehydes and ketones (as CH3COCH3)
≤0.005% carbonyl compounds (as CO)
≤0.01% tetrachloroethene (GC)
≤0.01% tetrachloromethane (GC)
≤0.01% trichloroethene (GC)
≤0.01% water (Karl Fischer)
≤0.03% dichloromethane (GC)
0.6-1.0% ethanol (GC)

refractive index

n20/D 1.445 (lit.)

bp

60.5-61.5 °C (lit.)

mp

−63 °C (lit.)

density

1.476-1.483 g/mL at 20 °C
1.492 g/mL at 25 °C (lit.)

anion traces

chloride (Cl-): ≤1 mg/kg

cation traces

Al: ≤0.5 mg/kg
B: ≤0.02 mg/kg
Ba: ≤0.1 mg/kg
Ca: ≤0.5 mg/kg
Cd: ≤0.05 mg/kg
Co: ≤0.02 mg/kg
Cr: ≤0.02 mg/kg
Cu: ≤0.02 mg/kg
Fe: ≤0.1 mg/kg
Mg: ≤0.1 mg/kg
Mn: ≤0.02 mg/kg
Ni: ≤0.02 mg/kg
Pb: ≤0.05 mg/kg
Sn: ≤0.1 mg/kg
Zn: ≤0.1 mg/kg

suitability

complies for appearance
complies for reaction against H2SO4
complies for suitability of determ. w. dithizone

Quality Level

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Application

Chloroform may be employed as a solvent for the following studies:
  • Extraction of RNA from plant cells.
  • Preparation of dioleoylphosphatidylcholine (DOPC) solution.
  • Dissolution of 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC).
It may be employed in a rapid quantitative method for the release of periplasmic proteins from bacterial strains.
Chloroform may be used as a solvent to enhance the enantioselectivity of secondary alcohols formed by the borohydride reduction of carbonyl compounds in the presence of optically active ketoiminatocobalt complexes.

General description

Chloroform, a halogenated hydrocarbon, is widely employed as a solvent. Its photochemical degradation has been investigated in the presence of TiO2 aqueous suspensions (wavelength range of 310-380nm).

Other Notes

For information on chloroform miscibility, please visit the following link:
Chloroform Miscibility/Immiscibility Table
The article number 32211-4X2.5L will be discontinued. Please order the single bottle 32211-2.5L which is physically identical with the same exact specifications.
The article number 32211-4X2.5L-M will be discontinued. Please order the single bottle 32211-2.5L-M which is physically identical with the same exact specifications.
The article number 32211-6X1L will be discontinued. Please order the single bottle 32211-1L which is physically identical with the same exact specifications.
The article number 32211-6X1L-M will be discontinued. Please order the single bottle 32211-1L-M which is physically identical with the same exact specifications.

pictograms

Skull and crossbonesHealth hazard

signalword

Danger

Hazard Classifications

Acute Tox. 3 Inhalation - Acute Tox. 4 Oral - Carc. 2 - Eye Irrit. 2 - Repr. 2 - Skin Irrit. 2 - STOT RE 1 Oral - STOT SE 3

target_organs

Central nervous system, Liver,Kidney

Classe de stockage

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

wgk

WGK 3

flash_point_f

does not flash

flash_point_c

does not flash


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Consulter la Bibliothèque de documents

Chloroform is not solvent but activator for cobalt complex catalyst of enantioselective borohydride reduction.
Kokura, Ai, et al.
Chemistry Letters (Jpn), 36(1), 26-27 (2006)
G F Ames et al.
Journal of bacteriology, 160(3), 1181-1183 (1984-12-01)
We introduce a method by which periplasmic proteins can be released rapidly, simply, and quantitatively by treating cells with chloroform. All the amino acid-binding proteins tested maintained their activity during chloroform treatment. This method makes practical the analysis of the
Jameel A Feshitan et al.
Journal of colloid and interface science, 329(2), 316-324 (2008-10-28)
Microbubbles used as contrast agents for ultrasound imaging, vectors for targeted drug delivery and vehicles for metabolic gas transport require better size control for improved performance. Mechanical agitation is the only method currently available to produce microbubbles in sufficient yields
Z V Leonenko et al.
Biochimica et biophysica acta, 1509(1-2), 131-147 (2000-12-19)
We have used magnetic alternating current mode atomic force microscopy (MAC-AFM) to investigate the formation of supported phospholipid bilayers (SPB) by the method of vesicle fusion. The systems studied were dioleoylphosphatidylcholine (DOPC) on mica and mica modified with 3-aminopropyl-triethoxy-silane (APTES)
Gurman Singh Pall et al.
Nucleic acids research, 35(8), e60-e60 (2007-04-05)
The northern blot, or RNA gel blot, is a widely used method for the discovery, validation and expression analysis of small regulatory RNA such as small interfering RNA (siRNA), microRNA (miRNA) and piwi-interacting RNA (piRNA). Although it is straightforward and

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