D3812
DirectLoad™ 50 bp DNA Step Ladder
ready-to-use marker for DNA electrophoresis
Synonyme(s) :
DirectLoad™ 50 bp DNA Marker, Ready-to-use 50 bp DNA Marker, Ready-to-use DirectLoad™ 50 bp DNA Marker, Ready-to-use DirectLoad™ 50 bp DNA Step Ladder
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A propos de cet article
NACRES:
NA.25
UNSPSC Code:
41105335
Service technique
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Laissez-nous vous aiderQuality Level
form
(liquid)
usage
sufficient for 100 loads
suitability
suitable for electrophoresis (DNA)
storage temp.
−20°C
General description
Sigma′s DirectLoad™ 50 bp DNA Step Ladder contains 17 blunt-ended fragments consisting of 50 bp repeats from 50 to 500 bp, 100 bp repeats from 600 to 900 bp, and 1 kb repeats from 1 to 3 kb. Ten ul of the ladder can be loaded directly in a single lane on an agarose or polyacrylamide gel.
Application
Suitable for size determination of PCR-generated DNA fragments with either agarose or polyacrylamide gels.
DirectLoad™ 50 bp DNA Step Ladder has been used for cell lysis and genomic cleavage detection assay.
Features and Benefits
- Ready-to-load
- Easy-to-use
- Popular band sizes
Other Notes
For optimal resolution, the recommended agarose gel concentration is 2.0%.
Sigma′s DirectLoad™ 50 bp DNA Step Ladder is provided in a gel-loading solution of 5% glycerol, 4.2 mM EDTA, 0.083% orange G and 0.0125% xylene cyanol.
Legal Information
DirectLoad is a trademark of Sigma-Aldrich Co. LLC
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Classe de stockage
12 - Non Combustible Liquids
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Contenu apparenté
Datasheet
Cinzia Fabbro et al.
FEMS microbiology letters, 307(2), 158-164 (2010-04-24)
Culturable vibrios were isolated from seawater collected during an annual sampling study performed along the Gulf of Trieste coast (Northern Adriatic Sea), and conventional culturing and identification methods were used to investigate the presence of Vibrio parahaemolyticus. Biochemically selected Vibrio
Solid-phase reverse transfection for intracellular delivery of functionally active proteins
Bulkescher R, et al.
Genome Research, 27(10), 1752-1758 (2017)