D7058
DirectLoad™ Wide Range DNA Marker
ready-to-use marker for DNA electrophoresis
Synonyme(s) :
agarose gel electrophoresis marker, gel ladder, gel marker, wide range ladder
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A propos de cet article
NACRES:
NA.25
UNSPSC Code:
41105335
Form:
liquid
Solubility:
water: soluble
Storage temp.:
−20°C
Service technique
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Laissez-nous vous aiderQuality Level
form
liquid
usage
100 uses
solubility
water: soluble
suitability
suitable for electrophoresis (DNA)
storage temp.
−20°C
General description
Sigma′s DirectLoad Wide Range DNA Marker contains 16 fragments from 50 – 10,000 bp, with bands regularly spaced. Ten ul of the ladder can be loaded directly in a single lane on an agarose or polyacrylamide gel.
Application
Suitable for size determination of PCR-generated DNA fragments with either agarose or polyacrylamide gels.
Features and Benefits
- Ready-to-load
- Easy-to-use
- Popular band sizes
Other Notes
Contains 16 fragments, 50–10,000 bp which produce logically spaced bands on an agarose gel.
For optimal resolution, the recommended agarose gel concentration is 0.75 – 2.0%.
Sigma′s DirectLoad™ Wide Range DNA Marker is provided in a gel-loading solution of 2.5% Ficoll (Type 400), 0.0125% bromophenol blue, and 0.00625% xylene cyanol.
Legal Information
DirectLoad is a trademark of Sigma-Aldrich Co. LLC
Classe de stockage
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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Contenu apparenté
Melanie P Dautle et al.
Journal of clinical microbiology, 40(2), 414-421 (2002-02-05)
In this study, 83 clinical isolates purified from biofilms colonizing 18 silicone gastrostomy devices (12 "buttons" and six tubes converted to skin level devices) were selected for subtype characterization utilizing genetic analysis. The tubes, previously used for feeding, remained in