M5755
MOPS-EDTA-Sodium Acetate (MESA)
powder for RNA electrophoresis buffers
Synonyme(s) :
MESA Buffer
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A propos de cet article
NACRES:
NA.25
UNSPSC Code:
12161700
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Molecular Biology
Quality Level
product line
BioReagent
form
powder
technique(s)
electrophoresis: suitable
pH
6.8-7.2(reconstituted per directions)
solubility
H2O: soluble, faintly yellow
foreign activity
DNAses, RNases, NICKases, endonucleases, and exonucleases, none detected
storage temp.
room temp
General description
MOPS – EDTA – Sodium Acetate (MESA) is the most commonly used running buffer for RNA electrophoresis prior to Northern blotting procedures. Applied voltage of 5 V/cm is recommended for maximum resolution.
Application
Suitable for use when making formaldehyde-agarose gels and associated running buffer for RNA electrophoresis.
Preparation Note
1x MESA buffer contains:
- 40 mM MOPS
- 10 mM sodium acetate
- 1 mM EDTA (pH 8.3)
Dissolve entire contents of bottle in a final volume of 1L molecular biology grade water. This produces a 10x MESA stock solution that can be further diluted as needed.
Classe de stockage
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Contenu apparenté
Sambrook, J., et al.
Molecular Cloning: A Laboratory Manual, 7-7 (1989)
Molecular techniques for detection of gene expression in neuroimmunology.
Cowan, E.P., and Dhib-Jalbut, S.S.
Methods in Neuroscience, 24, 41-41 (1995)
Kathryn M Jones et al.
Journal of bacteriology, 184(9), 2491-2499 (2002-04-12)
Two operons have been cloned from Anabaena sp. strain PCC 7120 DNA, each of which encodes the three core subunits of distinct mitochondrial-type cytochrome c oxidases. The two operons are only 72 to 85% similar to one another at the