S0902
SSC Buffer
for Northern and Southern blotting, powder blend
Synonyme(s) :
Saline-sodium citrate buffer
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A propos de cet article
NACRES:
NA.25
UNSPSC Code:
41105319
Service technique
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form
powder blend
pH
6.8-7.2 (20-25 °C, 263 mg/L in water)
solubility
water: 263 mg/mL, clear, colorless
foreign activity
DNAse, none detected, Endonuclease, none detected, Exonuclease, none detected, NICKase, none detected, RNAse, none detected
storage temp.
room temp
General description
SSC Buffer 20x Powder is a standard reagent in Southern and Northern blotting procedures. This concentrated powder should be reconstituted in molecular biology grade water to the indicated final volume before use (final solution is a 20x). The 20x concentrate can be used straight or diluted to make SSC working solutions with concentrations as low as 0.5x.
Application
Saline-sodium citrate (SSC) Buffer has been used as a washing buffer in microarray technique. It has also been used as a constituent of fluorescence in situ hybridization (FISH) buffer.
Suitable for:
- Preparing nucleic acids for hybridization
- Hybridization buffer for Northern and Southern blots
- Washing buffer for Northern and Southern blots
Features and Benefits
- Multi-purpose use in hybridizations
- Easily reconstituted and diluted to any concentration needed
Preparation Note
Final SSC Buffer 20x Concentrate contains 0.3 M sodium citrate in 3M NaCl.
Classe de stockage
13 - Non Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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Nucleic acids research, 9(20), 5253-5268 (1981-10-24)
Complexes of histones H1 with superhelical SV40 DNA obtained by direct mixing were studied in 0.1 SSC buffer corresponding to 0.02 M Na+. Depending on the molar input ratio H1/DNA three classes of sedimenting species were observed: (1) a component