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Merck

A25120

DEAE-Sephadex® A-25 chloride form

Synonyme(s) :

DEAE Sephadex®, Sephadex A 25,2-(diethylamino)ethylether, Diethylaminoethyl Sephadex®

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A propos de cet article

Numéro CAS:
12609-80-2
UNSPSC Code:
47101511
NACRES:
NA.56
MDL number:
MFCD00132778

Principaux documents

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form

powder

technique(s)

activity assay: suitable

matrix

cross-linked dextran

matrix active group

functional group

bead size

40-125 μm (dry)

pore size

~30,000 Da exclusion limit

operating pH

2-9

capacity

3-4 meq/g ion exchange capacity

compatibility

mode of use mixed weak and strong anion exchange chromatography

Quality Level

200

General description

The Sephadex® ion exchangers contain a cross-linked dextran matrix with functional groups. These groups are linked by stable ether linkages to the glucose units in the matrix. DEAE Sephadex can be used in ion exchange chromatography for purifying and isolating proteins.

A25120-100G′s updated product number is GE17-0170-01
A25120-500G′s updated product number is GE17-0180-02

Application

DEAE-Sephadex® A-25 has been used:
  • to form DEAE-Sephadex A-25 (formate form) for glucosinolates (GLS) extraction using horseradish plants
  • in the estimation of GLS content in Chinese kale sprouts
  • production and purification of endozepine from astrocytes
  • to identify and label an insulin-activated nitric oxide synthase inhibitor (protein) in acute myocardial infarction (AMI) patients

Features and Benefits

  • Weak anion exchanger
  • Suitable for batch techniques
  • High binding capacities
  • Non-reactive to commonly used aqueous buffers additives such as 8 M urea and 6 M guanidine hydrochloride

Legal Information

DEAE Sephadex is a registered trademark of Cytiva
Sephadex is a registered trademark of Cytiva

Classe de stockage

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificats d'analyse (COA)

Lot/Batch Number
0000514869
0000514868
0000514869
0000514868
0000486762

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How should I store Product A25120, DEAE-Sephadex® A-25 chloride?

    Unused media can be stored in the container at +4 to +30 °C.  Ensure that the screw-top is fully tightened.  Packed columns should be equilibrated in working buffer containing 20% ethanol to prevent microbial growth.

  4. What is the chemical stability of Product A25120, DEAE-Sephadex® A-25 chloride form? 

    According to the manufacturer, this is compatible with all commonly used aqueous buffers, 1 M NaOH, 8 M urea, 6 M guanidine hydrochloride and 70% ethanol.

  5. How do I equilibrate Product A25120, DEAE-Sephadex® A-25 chloride form?

    After packing, and before a chromatographic run, equilibrate with working buffer by washing with at least 5 bed volumes.

  6. How should I regenerate Product A25120, DEAE-Sephadex® A-25 chloride form?

    After each separation, elute any reversibly bound material either with a high ionic strength solution (e.g. 1 M NaCl in buffer) or by increasing pH. Regenerate the media by washing with at least 5 bed volumes of buffer, or until the column effluent shows stable conductivity and pH values.

  7. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  8. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  9. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

T M Parkinson
Journal of lipid research, 8(1), 24-29 (1967-01-01)
Various cellulose and dextran anion exchangers bind bile salts in vitro under conditions of pH and ionic strength resembling those in the lumen of the small intestine. Of these substances, diethylaminoethyl (DEAE) cellulose, guanidoethyl cellulose, and DEAE Sephadex reduced hypercholesterolemia
Human Lymphocyte Agglutinins in <I>Tursiops Truncatus</I> (Bottlenose Dolphin).
Hohn, D., et al.
Immunological Investigations, 12(5), 481-490 (1983)
A C Newton et al.
Analytical biochemistry, 156(1), 56-60 (1986-07-01)
The greatly enhanced fluorescence of Tb3+ when complexed with dipicolinic acid affords a simple and highly sensitive method for monitoring continuous anion flux through the erythrocyte anion transporter, band 3. Dipicolinic acid (pyridine-2,6-dicarboxylic acid) is entrapped in human erythrocytes and
In vitro comparison of embryonic myoblasts and myogenic cells isolated from regenerating adult rat skeletal muscle.
P H Jones
Experimental cell research, 139(2), 401-404 (1982-06-01)
H Wang et al.
Neuroscience, 46(4), 793-806 (1992-01-01)
We previously isolated two glutamate-binding proteins from rat brain synaptic membranes of 71,000 and 63,000 mol. wt [Chen et al. (1988) J. biol. Chem. 263, 417-426]. In the present study, the 71,000 and 63,000 mol. wt glutamate-binding proteins were purified
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