A3687
Conjugué anticorps anti-IgG de lapin (molécule entière)–phosphatase alcaline antibody produced in goat
affinity isolated antibody, buffered aqueous glycerol solution
Synonyme(s) :
Alkaline Phosphatase Secondary Antibody, Anti Rabbit Antibody - Anti-Rabbit IgG (whole molecule)–Alkaline Phosphatase antibody produced in goat, Anti Rabbit Sigma, anti rabbit antibody
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A propos de cet article
Conjugate:
alkaline phosphatase conjugate
Clone:
polyclonal
Application:
ELISA (d), IHC (p), WB
Species reactivity:
rabbit
Citations:
339
Technique(s):
direct ELISA: 1:30,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50, western blot: 1:30,000
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Laissez-nous vous aiderbiological source
goat
conjugate
alkaline phosphatase conjugate
antibody form
affinity isolated antibody
antibody product type
secondary antibodies
clone
polyclonal
form
buffered aqueous glycerol solution
species reactivity
rabbit
technique(s)
direct ELISA: 1:30,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50, western blot: 1:30,000
shipped in
wet ice
storage temp.
2-8°C
target post-translational modification
unmodified
Quality Level
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Application
La citrullination de l'antithrombine par PADI4 a été analysée par ELISA à l'aide d'anticorps de chèvre anti-IgG de lapin conjugué à la phosphatase alcaline comme anticorps secondaire, dilué au 1/5 000e dans un tampon carbonate-bicarbonate 0,05 M (pH 9,6).
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Dot blot (1 paper)
Enzyme-linked immunosorbent assay (1 paper)
Immunohistochemistry (1 paper)
Western Blotting (10 papers)
Dot blot (1 paper)
Enzyme-linked immunosorbent assay (1 paper)
Immunohistochemistry (1 paper)
Western Blotting (10 papers)
Goat anti-Rabbit IgG (whole molecule)-Alkaline Phosphatase antibody has been used for western blot and ELISA applications.the antibody can also be used for immunohistochemistry (at 1:50, using formalin-fixed, paraffin-embedded sections).
The level of hypoxyprobe binding proteins present in retinal mouse tissue was analyzed by ELISA using alkaline phosphatase conjugated goat anti-rabbit IgG as the secondary at a dilution of 1:2000 in 5% tween/PBS. Substrate used was 4-nitrophenyl phosphate disodium salt hexahydrate (Sigma).
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
IgGs are glycoprotein antibodies that modulate several immune responses. Rabbit IgGs against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-rabbit IgGs conjugated to a detectable substrate are useful tools for the analysis of target proteins. Goat anti-Rabbit IgG (whole molecule)-Alkaline Phosphatase antibody binds all rabbit Igs.
Immunogen
purified rabbit IgG as the immunogen
Physical form
Solution in 0.05 M Tris buffer, pH 8.0, containing 1 mM MgCl2, 10 mM glycine, 1% bovine serum albumin, 50% glycerol and 15 mM sodium azide.
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Sônia de Fátima Soto et al.
PloS one, 12(8), e0183314-e0183314 (2017-08-19)
Female Wistar rats were exposed to filtered air (F) or to concentrated fine particulate matter (P) for 15 days. After mating, the rats were divided into four groups and again exposed to F or P (FF, FP, PF, PP) beginning
Rod photoreceptor loss in Rho-/- mice reduces retinal hypoxia and hypoxia-regulated gene expression.
Tanyth E de Gooyer et al.
Investigative ophthalmology & visual science, 47(12), 5553-5560 (2006-11-24)
This study was conducted to evaluate whether regions of the retinal neuropile become hypoxic during periods of high oxygen consumption and whether depletion of the outer retina reduces hypoxia and related changes in gene expression. Retinas from rhodopsin knockout (Rho-/-)
Xianwen Zhang et al.
PloS one, 9(12), e115459-e115459 (2014-12-23)
Using transgenic rice as a bioreactor for mass production of pharmaceutical proteins could potentially reduce the cost of production significantly. However, a major concern over the bioreactor transgenic rice is the risk of its unintended spreading into environment and into
Pingxi Xiao et al.
Experimental and therapeutic medicine, 14(4), 3812-3816 (2017-10-19)
Rheumatic heart disease (RHD) occurs due to the accumulation of complications associated with rheumatic fever, and it results in high morbidity and mortality. The majority of cases of RHD are diagnosed in the chronic stages, when treatment options are limited.
P Manders et al.
British journal of cancer, 87(7), 772-778 (2002-09-17)
The growth and metastasising capacity of solid tumours are dependent on angiogenesis. Vascular endothelial growth factor is a mediator of angiogenesis. In this study we investigated whether vascular endothelial growth factor is associated with the natural course of the disease
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