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Merck

A5060

Anti-Actin (20-33) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonyme(s) :

Anti-ACTA

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A propos de cet article

UNSPSC Code:
12352203
NACRES:
NA.46
MDL number:
MFCD01092261

Principaux documents

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Nom du produit

Anti-Actin (20-33) antibody produced in rabbit, IgG fraction of antiserum, buffered aqueous solution

biological source

rabbit

conjugate

unconjugated

antibody form

IgG fraction of antiserum

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 42 kDa

species reactivity

wide range (animal), invertebrates

packaging

antibody small pack of 25 μL

enhanced validation

independent
Learn more about Antibody Enhanced Validation

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable using chicken or rat tissue sections
indirect immunofluorescence: 1:200 using cultured human fibroblasts
microarray: suitable
western blot: 1:250 using using rat brain or chicken muscle extracts

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

200

Gene Information

human ... ACTA1(58)
mouse ... Acta1(11459)
rat ... Acta1(29437)

Catégories apparentées

Application

Homogenates from limb tissue of E13.5 mouse embryos were analyzed by western blot using rabbit anti-actin as a primary antibody at a dilution of 1:8000.
Rabbit Anti-Actin (20-33) antibody has been used for immunoblotting assays. The product can also be used for immunohistochemistry, indirect immunofluorescence and microarray analyses.

Biochem/physiol Actions

Actin maintains the cell shape and participates in cytokinesis. Actin provides cellular strength and promotes active movement of the cell. Actin protein is essential for the formation of mature platelets. Upregulation of ACTA1 (actin, α 1) gene is observed in orthotopic liver transplantation without any earlier evidence of autoimmune liver disease.
Anti-Actin recognizes an epitope located on the N-terminal region of actin. The antibody specifically labels actin in a wide variety of tissues and species using immunoblotting (band at 42kDa). It specifically stains typical stress fibers in cultured cells using indirect immunofluorescent staining and immunohistochemistry.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

A mature actin filament consists of a right-handed double helix made of two F-actin protofilaments.
The antibody shows a broad reactivity among actin isoforms and across a range of organisms. It specifically stains typical stress fibers in cultured cells using an indirect immunofluorescent labeling technique and can be used as a probe of the N-terminal region of actin.
Actin is a highly conserved protein that is a major component of both the cytoskeletal and contractile structures in all cell types. It varies in amount, being related to the type of differentiation and to the functional state of cells and tissues. Actin can be found in two different forms of aggregation, the globular or the fibrillar state, and at least six distinct isoforms occur in vertebrates. The actins exhibit over 90% sequence homology, but each isoform has a unique NH2-terminal sequence. The isoforms are comprised of three α actins (skeletal, cardiac, smooth), one β actin (β-non-muscle) and two γ actins (γ smooth muscle and γ non-muscle).

Immunogen

N-terminal actin peptide attached to a multiple antigen peptide (MAP) backbone.

Other Notes

To view an Actin antibody selection guide, please visit www.sigmaaldrich.com/actin.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide as preservative.

Preparation Note

For continuous use, store at 2-8 °C for a maximum of one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

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Classe de stockage

10 - Combustible liquids

wgk

WGK 2

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Certificats d'analyse (COA)

Lot/Batch Number
0000504549
0000504549
0000466583
0000466583
0000445859

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Consulter la Bibliothèque de documents

Cytoskeletal regulation of platelet formation: Coordination of F-actin and microtubules.
Poulter N S and Thomas S G
The International Journal of Biochemistry & Cell Biology, 66, 69-74 (2015)
Lamellipodin promotes actin assembly by clustering Ena/VASP proteins and tethering them to actin filaments.
Hansen S D, et al.
eLife, 4, e06585-e06585 (2015)
An actin-based cytoskeleton in archaea.
Ettema T J G, et al.
Molecular Microbiology, 80(4), 1052-1061 (2011)
Lack of Clinical Relevance of ANA and ASMA Positivity in Patients with Liver Transplantation without a History of Autoimmune Diseases.
Pellegrini L, et al.
BioMed Research International, 2017 (2017)
Kornelius Schulze et al.
Nature genetics, 47(5), 505-511 (2015-03-31)
Genomic analyses promise to improve tumor characterization to optimize personalized treatment for patients with hepatocellular carcinoma (HCC). Exome sequencing analysis of 243 liver tumors identified mutational signatures associated with specific risk factors, mainly combined alcohol and tobacco consumption and exposure
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