Anti-Aurora B antibody produced in rabbit

Auroa B is a member of a family of Auora kinases that function for proper chromosomal segregation during mitosis. Auora B localizes specifically to the kinetochores of microtubules.

Aurora B (AIRK2, AIR-2 kinase, AIM-1) is a serine/threonine kinase. Aurora B is evolutionally conserved from yeast to human. The Drosophila serine/threonine protein kinase Aurora and the S. cerevisiae IpI1 kinase are highly homologous to human Aurora B.

synthetic peptide corresponding to amino acid residues 1-19 of human Aurora B with C-terminal added cysteine conjugated to KLH.

Anti-Aurora B antibody has been used in:

• in immunoblotting
• in immunoprecipitation
• in immunofluorescence
• in immunofluorescence staining
• in western blotting

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Rabbit polyclonal anti-Aurora B antibody is used to tag Aurora B/AIR-2 Kinase for detection and quantitation by immunocytochemical and immunohistochemical (IHC) techniques. It is used as a probe to determine the presence and roles of Aurora B/AIR-2 Kinase in chromosome segregation, cytokinesis, and cancer development.

Anti-Aurora B recognizes human, mouse, and rat Aurora B/AIR-2 Kinase enzymes. Detection of the Aurora B band by immunoblotting is specifically inhibited with the immunizing peptide.

Aurora B plays key roles in chromosome segregation, cytokinesis, and cancer development. It also plays a role in chromosomal condensation by phosphorylating the H3 histone. In C. elegans, Aurora-B is required for normal localization and function of the ZEN-4/CeMKLP (Kinesin like protein/mitotic kinesin-like protein), a kinesin-related protein essential for completion of cytokinesis. Loss of the Aurora B kinase results in chromosome segregation defects and failures in cytokinesis. Aurora B displays a localization pattern typical of chromosomal passenger proteins as the inner centromeric protein (INCENP), TD-60 and Survivin. INCENP and Survivin interact directly with Aurora B. Chromosomal passenger proteins undergo dynamic redistribution during mitosis. They localize at centromers during prometaphase and relocate to midzone microtubules and midbodies during anaphase and telophase. The mRNA and protein levels of Aurora B are induced during G₂M and decrease rapidly after the end of mitosis. Levels of Aurora B are increased in several human cancer cell lines.

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

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