A6410
Apyrase from potatoes
ATPase ≥200 units/mg protein, lyophilized powder
Synonyme(s) :
Adénosine 5′-diphosphatase, Adénosine 5′-triphosphatase
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A propos de cet article
Numéro CAS:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-569-8
MDL number:
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Grade VI
form
lyophilized powder
ATPase activity
≥200 units/mg protein
secondary activity
≤15 % of base activity ADPase
mol wt
~49 kDa by gel filtration
composition
Protein, 30-60%
solubility
H2O: soluble 1.0 mg/mL
foreign activity
Acid Phosphatase ≤2% of base activity
storage temp.
−20°C
Quality Level
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General description
Apyrase, also known as ATP-diphosphohydrolase, is expressed in wide variety of plant and animal tissues. The commercially accessible apyrase enzyme is isolated from potato tubers.
Application
Au moins deux isoenzymes sont présentes dans diverses souches de S. tuberosum : l′une avec un rapport ATPase/ADPase élevé (∼ 10) et l′autre avec un rapport faible (∼ 1).
Réaction : ATP → ADP+Pi → AMP+2Pi.
Réaction : ATP → ADP+Pi → AMP+2Pi.
Apyrase from potatoes has been used:
- to deplete ATP
- to degrade endogenous extracellular ATP
- as a supplement in acid citrate dextrose solution to treat blood obtained from anesthetized mice
Apyrase is used to hydrolyze nucleoside triphosphates and diphosphates. For hydrolysis of organic di and triphosphates, the optimal pH is 6, and for inorganic substrates, the optimal pH is 5.1. Apyrase, from Sigma, has been used in inhibition studies of platelet-aggregation .
Biochem/physiol Actions
Apyrase hydrolyses the phosphoanhydride bonds of nucleoside tri- and di-phosphates in the presence of divalent cations. It has wide substrate specificity for nucleotides. This property of the enzyme makes it suitable for different biotechnical applications, including DNA sequencing and platelet-aggregation inhibition. Apyrase has adenosine 5′-triphosphatase (ATPase) and adenosine 5′-diphosphatase (ADPase) activities.
Apyrase is found in all eukaryotes and some prokaryotes. Apyrase, from potato, has a crucial role in regulating growth and development. Apyrase is involved in the inactivation of synaptic ATP as a neurotransmitter following nerve stimulation and in the inhibition of ADP induced platelet aggregation to prevent thrombosis . Divalent metal ions are required for activity and best activity is observed with calcium ion at 5 mM.
Packaging
Sold on the basis of ATPase units.
Physical form
Partially purified, lyophilized powder containing potassium succinate buffer salts.
Preparation Note
Derived from white potato
Other Notes
One unit will liberate 1.0 μmole of inorganic phosphate from ATP or ADP per min at pH 6.5 at 30 °C.
This grade has an exceptionally high ATPase/ADPase ratio.
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Classe de stockage
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Loop extrusion by structural maintenance of chromosomes (SMC) complexes has been proposed as a mechanism to organize chromatin in interphase and metaphase. However, the requirements for chromatin organization in these cell cycle phases are different, and it is unknown whether
Methylotrophic yeast Pichia pastoris as a host for production of ATP-diphosphohydrolase (apyrase) from potato tubers (Solanum tuberosum).
Nourizad N, et al.
Protein Expression and Purification, 27(2), 229-237 (2003)
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