A7437
Acetate Kinase from Escherichia coli
lyophilized powder, ≥150 units/mg protein (biuret)
Synonyme(s) :
ATP:Acetate phosphotransferase
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A propos de cet article
Numéro CAS:
UNSPSC Code:
12352204
eCl@ss:
32160410
NACRES:
NA.54
MDL number:
Specific activity:
≥150 units/mg protein (biuret)
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lyophilized powder
specific activity
≥150 units/mg protein (biuret)
composition
Protein, 5.0-20.0%
UniProt accession no.
foreign activity
adenosine triphosphatase and NADH oxidase ≤0.01%
storage temp.
−20°C
Quality Level
Gene Information
Escherichia coli K12 ... ackA(946775)
General description
Acetate kinase belongs to acetate and sugar kinase/Hsc70/actin (ASKHA) structural superfamily. It exists as a dimer and has nucleotide binding site in the cleft between N and C-terminal domain.
Application
Acetate Kinase from Escherichia coli has been used in assessing acetate kinase activity during the fermatation process of Gluconacetobacter xylinus and in kinetic modeling and steady state studies.
Acetate kinase is used to phosphorylate acetate to acetyl phosphate. Acetate Kinase from Escherichia coli has been used as part of an ATP-regenerating system to study the kinetics of agonist-stimulated transphosphatidylation .
Biochem/physiol Actions
Acetate kinase plays an important role in glycolysis. Acetate kinase phosphorylates acetate in the presence of ATP and a divalent cation, which ultimately results in the production of acetyl-CoA . Acetate kinase is also involved in the metabolism of propanoate, pyruvate and taurine.
Involved in the metabolism of propanoate, pyruvate and taurine
Physical form
Lyophilized powder containing trehalose with small amounts of potassium phosphate, magnesium chloride, and dithiothreitol
Other Notes
One unit will phosphorylate 1.0 μmole of acetate to acetyl phosphate per min at pH 7.6 at 25 °C.
Classe de stockage
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Influence of residual ethanol concentration on the growth of Gluconacetobacter xylinus I 2281
Kornmann H, et al.
Applied Microbiology and Biotechnology, 62(2-3), 168-173 (2003)
S C Olson et al.
The Journal of biological chemistry, 266(26), 17236-17242 (1991-09-15)
Receptor-linked activation of phospholipase D has been demonstrated recently in a variety of intact cell types including granulocytes, but little is known about the enzyme, its cofactor requirements, and regulation. Using [3H]alkyllysophosphatidylcholine to prelable an endogenous phosphatidylcholine substrate pool in
Urkinase: structure of acetate kinase, a member of the ASKHA superfamily of phosphotransferases
Buss KA, et al.
Journal of Bacteriology, 183(2), 680-686 (2001)
Hélène Scornec et al.
Frontiers in microbiology, 11, 156-156 (2020-03-21)
The cell surface is the primary recognition site between the bacterium and the host. An operon of three genes, LSEI_0219 (cwaR), LSEI_0220 (cwaS), and LSEI_0221 (ldcA), has been previously identified as required for the establishment of Lactobacillus paracasei in the
The role of the C8 proton of ATP in the regulation of phosphoryl transfer within kinases and synthetases
Kenyon CP, et al.
BMC Biochemistry, 12(1), 36-36 (2011)
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