A7510
Adenosine 5′-Triphosphatase from porcine cerebral cortex
lyophilized powder, ≥0.3 units/mg protein, pH 7.8
Synonyme(s) :
ATP phosphohydrolase, ATPase
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A propos de cet article
Numéro CAS:
UNSPSC Code:
12352204
NACRES:
NA.54
MDL number:
Specific activity:
≥0.3 units/mg protein, pH 7.8
Biological source:
Porcine cerebral cortex
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Laissez-nous vous aiderbiological source
Porcine cerebral cortex
description
Sodium and Potassium-Activated
form
lyophilized powder
specific activity
≥0.3 units/mg protein, pH 7.8
composition
Protein, 5.0-25.0% biuret
technique(s)
inhibition assay: suitable
storage temp.
−20°C
Quality Level
Gene Information
pig ... ATP1A1(397481), ATP1B1(396898)
Application
ATPase is used to liberate inorganic phosphorus from ATP. ATPase, from porcine cerebral cortex, has been used in inhibition assays of Na/K-ATPase activity .
Biochem/physiol Actions
This membrane enzyme hydrolyzes ATP to ADP and orthophosphate. The hydrolysis is coupled with the exchange of sodium and potassium ions across the plasma membrane. The enzyme is present in almost all tissues of higher organisms, but it is most abundant in the kidney where it reabsorbs sodium ions from the glomerular filtrate. Na+,K+-ATPase is affected by changes in intracellular calcium. It is also sensitive to ouabain and inhibited by vanadate, and (in vitro) by SPAI-1, a peptide isolated from porcine duodenum. It has been reported to be regulated by phosphorylation by protein kinase A (PKA). Bufalin, and other cardiotonic steroids, may inhibit Na+/K+-ATPase activity and induce the differentiation of HL60, U937, and ML1 cells .
Other Notes
One unit will liberate 1.0 μmole of inorganic phosphorus from ATP per min at pH 7.8 at 37 °C in the presence of Na+, K+, and Mg2+.
Physical form
Lyophilized powder containing 90% sucrose, 0.4% EDTA Na4 and 0.06% NaCl
Preparation Note
Sodium iodide extracted fraction of Nakao, et al., Biochem. Biophys. Res. Commun., 19, 755 (1965).
Classe de stockage
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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