A7646
Apyrase from potatoes
ATPase ≥60 units/mg protein, lyophilized powder (partially purified)
Synonyme(s) :
Adénosine 5′-diphosphatase, Adénosine 5′-triphosphatase
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A propos de cet article
Numéro CAS:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-569-8
MDL number:
Biological source:
potato
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Laissez-nous vous aiderbiological source
potato
type
Grade III
form
lyophilized powder (partially purified)
ATPase activity
≥60 units/mg protein
mol wt
~49 kDa by gel filtration
composition
Protein, 30-60%
solubility
H2O: soluble 10 mg/mL, slightly hazy
application(s)
diagnostic assay manufacturing
foreign activity
Acid Phosphatase ≤5% of base activity
storage temp.
−20°C
Quality Level
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Application
Au moins deux isoenzymes sont présentes dans diverses souches de S. tuberosum : l′une avec un rapport ATPase/ADPase élevé (∼ 10) et l′autre avec un rapport faible (∼ 1).
Réaction : ATP → ADP+Pi → AMP+2Pi.
Réaction : ATP → ADP+Pi → AMP+2Pi.
Apyrase is used to hydrolyze nucleoside triphosphates and diphosphates. For hydrolysis of organic di and triphosphates, the optimal pH is 6, and for inorganic substrates, the optimal pH is 5.1 Apyrase, from Sigma, has been used in inhibition studies of platelet-aggregation. The enzyme has been used to assess the possibility of ATP involvement in intestinal lamina propria Th17 cell differentiation.
Biochem/physiol Actions
Apyrase is found in all eukaryotes and some prokaryotes. Apyrase, from potato, has a crucial role in regulating growth and development. Apyrase is involved in the inactivation of synaptic ATP as a neurotransmitter following nerve stimulation and in the inhibition of ADP induced platelet aggregation to prevent thrombosis. Divalent metal ions are required for activity and best activity is observed with calcium ion at 5 mM. The molecular weight of the protein is found to be approximately 45 kDa.
Packaging
Sold on the basis of ATPase units
Analysis Note
Enthält Säurephosphatase.
Other Notes
Mixture of high and low ratio isoenzymes.
One unit will liberate 1.0 μmole of inorganic phosphate from ATP or ADP per min at pH 6.5 at 30 °C.
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Classe de stockage
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Marko Gosak et al.
Life (Basel, Switzerland), 11(5) (2021-05-01)
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Journal of tissue engineering and regenerative medicine, 13(12), 2155-2168 (2019-09-11)
This study tested the hypothesis that preactivated and disaggregated shape-changed platelet (PreD-SCP) therapy significantly protected rat kidney from ischemia-reperfusion (IR) injury. Adult-male Sprague-Dawley rats (n = 24) were equally categorized into Groups 1 (sham-operated control [SC]), 2 (SC + PreD-SCP)
H J Kruse et al.
Arteriosclerosis and thrombosis : a journal of vascular biology, 14(11), 1821-1828 (1994-11-01)
The blocker of receptor-mediated calcium entry SK&F 96365 was used to evaluate the contribution of calcium influx to the formation of biologically active endothelial prostanoids and endothelium-derived relaxing factor (EDRF). SK&F 96365 inhibited histamine-stimulated calcium entry into human umbilical vein
Properties of Two Apyrases from Solanum tuberosum
Kettlun, A. et al.
Phytochemistry, 21(3), 551-558 (1982)
Xiao-Jing Kuang et al.
Theranostics, 12(8), 3703-3718 (2022-06-07)
Rationale: Stress is a major risk factor for the development of depression. However, the underlying molecular mechanisms of stress vulnerability in depression are largely uncharacterized. Methods: P2X2 receptors (a major receptor for gliotransmitter-ATP) in the medial prefrontal cortex (mPFC) were
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