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Merck

A8781

β-Amylase from sweet potato

greener alternative

Synonyme(s) :

1,4-α-D-Glucan maltohydrolase

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A propos de cet article

Numéro CAS:
9000-91-3
UNSPSC Code:
41106514
NACRES:
NA.56
EC Number:
232-566-1
MDL number:
MFCD00081391
Numéro CE :
3.2.1.2 (BRENDA, IUBMB)

Principaux documents

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form

powder

mol wt

~200,000

packaging

vial of 15 mg

greener alternative product score

old score: 57
new score: 1
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greener alternative product characteristics

Waste Prevention
Safer Solvents and Auxiliaries
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

greener alternative category

Re-engineered,

storage temp.

−20°C

Quality Level

100

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Catégories apparentées

Application

β-Amylase from sweet potato has been used:
  • as a molecular weight marker in blue native polyacrylamide gel electrophoresis (PAGE)
  • as a standard for calibration curve preparation in size exclusion chromatography
  • as a model protein for small-angle X-ray analysis (SAXs)

Biochem/physiol Actions

β-Amylase acts on the polysaccharide α(1,4) glycosidic linkages and releases maltose present in the non-reducing ends. Due to its stability, β-Amylase from sweet potato is used in the immobilized enzyme preparation in combination with agarose beads.

General description

β-Amylase from sweet potato is a tetrameric enzyme with each monomer comprising (α/β) core domain and a C-terminal loop region. It belongs to the glycoside hydrolase family GH14.
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pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Classe de stockage

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

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Certificats d'analyse (COA)

Lot/Batch Number
0000290697
0000290697
SLCQ9616
SLCQ9616
SLCP3236

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Consulter la Bibliothèque de documents

Integration of affinity precipitation with partitioning methods for bioseparation of chitin binding lectins.
Teotia, S. et al.
Food and Bioproducts Processing, 84, 37-43 (2006)
S Teotia et al.
Enzyme and microbial technology, 28(9-10), 792-795 (2001-06-09)
beta-amylases are used in production of maltose syrup. It is shown that sweet potato beta-amylase can be purified by affinity precipitation with alginate with 80% activity yield and 44 fold purification. SDS-PAGE of the purified protein showed a single band
Liyu Tu et al.
Journal of cell science, 119(Pt 24), 5077-5086 (2006-12-13)
The surface of the mammalian urinary bladder is covered by a crystalline, asymmetric unit membrane (AUM) structure that contains the four major uroplakins (UPs): Ia, Ib, II and IIIa. UPIa and UPIb belong to the family of tetraspanins. Although UPIa
Purification of staphylococcal protein A by affinity precipitation using a reversibly soluble-insoluble polymer with human IgG as a ligand
Taniguchi, M. et al.
Journal of Fermentation and Bioengineering, 68, 32-36 (1989)
Khalilah G Reddie et al.
Molecular bioSystems, 4(6), 521-531 (2008-05-22)
Oxidation of the thiol functional group in cysteine (Cys-SH) to sulfenic (Cys-SOH), sulfinic (Cys-SO2H) and sulfonic acids (Cys-SO3H) is emerging as an important post-translational modification that can activate or deactivate the function of many proteins. Changes in thiol oxidation state
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