APOAC
Annexin V-Cy3™ Apoptosis Detection Kit
Synonyme(s) :
Red fluorescent Annexin V apoptosis assay
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A propos de cet article
NACRES:
NA.32
UNSPSC Code:
12352207
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Laissez-nous vous aiderusage
sufficient for 200 tests
Quality Level
packaging
pkg of 1 kit
technique(s)
flow cytometry: suitable
application(s)
cell analysis
detection
detection method
fluorometric
shipped in
wet ice
storage temp.
2-8°C
Biochem/physiol Actions
Sigma′s Annexin V-Cy3 kit allows detection of annexin V bound to apoptotic cells by fluorescence microscopy. The Annexin V-Cy3 kit uses the dye Cy3.18 as the fluorochrome conjugated with annexin V. By microscopy, Cy3.18 fluoresces more brightly than the FITC conjugate. The kit includes the non-fluorescent compound 6-carboxyfluorescein diacetate (6-CFDA), which enters the cell and is hydrolyzed by the esterases present in living cells to the fluorescent compound 6-carboxyfluorescein, indicating that the cells are viable. This combination allows the differentiation among early apoptotic cells (annexin V positive, 6-CFDA positive), necrotic cells (annexin V positive, 6-CFDA negative), and viable cells (annexin V negative, 6-CFDA positive).
Features and Benefits
Detects apoptosis earlier in the process than DNA-based assays such as TUNEL
- Rapid labeling of cells. Cell staining takes only 10 minutes.
- No cell fixation or processing required, reducing the detection time and allowing the cells to be used for further study.
- 6-Carboxyfluorescein diacetate secondary dye is included with the kit to differentiate apoptotic cells from viable and necrotic cells.
Legal Information
Cy3 is a trademark of Cytiva
Composants de kit seuls
Réf. du produit
Description
- Annexin V Cy3.18 Conjugate from human placenta, buffered aqueous solution 10 μg
Composants de kit également disponibles séparément
Réf. du produit
Description
FDS
- C50416-Carboxyfluorescein diacetate 10 mg
Classe de stockage
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
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