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Merck

B0556

Anti-B23 antibody, Mouse monoclonal

clone FC82291, purified from hybridoma cell culture

Synonyme(s) :

Anti-NPM, Anti-Nucleophosmin

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A propos de cet article

UNSPSC Code:
12352203
NACRES:
NA.41
MDL number:
MFCD03457543

Principaux documents

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Nom du produit

Anti-B23 antibody, Mouse monoclonal, clone FC82291, purified from hybridoma cell culture

Quality Level

200

biological source

mouse

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

FC82291, monoclonal

form

buffered aqueous solution

mol wt

antigen 37 kDa

species reactivity

rat, kangaroo rat, canine, bovine, human, mouse, monkey, hamster

packaging

antibody small pack of 25 μL

concentration

~0.5 mg/mL

technique(s)

immunocytochemistry: suitable using 2% formaldehyde-acetone or 10% formalin/methanol-1% NP-40
immunoprecipitation (IP): suitable
indirect ELISA: suitable
western blot: 0.2-0.4 μg/mL using a whole extract of 3T3 (mouse fibroblasts) cells

isotype

IgG1

UniProt accession no.

P06748

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... NPM1(4869)
mouse ... Npm1(18148)
rat ... Npm1(25498)

Application

The antibody may be used for ELISA, competitive ELISA, immunoblotting (37 kDa), immunoprecipitation, immunocytochemistry (2% formaldehyde-acetone 1,2,5 or 10% formalin/methanol-1% NP-40 and microinjection (blocks the initiation of centrosome duplication) and immunohistochemistry. Reactivity has been observed with human, monkey, bovine, dog, hamster (weak), rat,kangaroo rat, and mouse B23.

Biochem/physiol Actions

It detects both the phosphorylated and the unphosphorylated B23 molecule. The epitope recognized by the antibody lies within the 68 amino acids at the C-terminus of B23.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

B23 (also known as NPM, nucleophosmin, numatrin or NO38) is a phosphoprotein, widely expressed in all cell types, and localized in granular regions of the nucleolus associated with pre-ribosomal particles. This protein was found to be involved in several nuclear functions such as assembly and/or intranuclear transport of preribosomal particles, cytoplasmic/nuclear trafficking, regulation of DNA polymerase A activity and centrosome duplication. Phosphorylation of B23 is mediated by a cyclin complex, CDK2/cyclin E.
Phosphorylation causes the dissociation of B23 from the centrosome and enables the duplication of the centrosome during cell mitosis. B23 was also found to be involved in pro-myelocytic leukemia in which the gene for B23 fuses with retinoic acid receptor a (RARa). As a consequence, a fusion protein (60-7 0 kDa) is produced containing parts of the two genes.

Immunogen

rat B23 (nucleophosmin, NPM, numatrin).

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide.

Preparation Note

Purified from culture supernatant of hybridoma cells grown in a bioreactor.

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Classe de stockage

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificats d'analyse (COA)

Lot/Batch Number
0000508934
0000508934
0000473282
0000473282
0000458109

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Consulter la Bibliothèque de documents

Dynamic localization of alpha-tubulin acetyltransferase ATAT1 through the cell cycle in human fibroblastic KD cells
Nekooki-Machida, et al.
Medical Molecular Morphology, 1-10 (2018)
Katharina Thanisch et al.
Nucleic acids research, 42(6), e38-e38 (2013-12-29)
Epigenetic regulation of gene expression involves, besides DNA and histone modifications, the relative positioning of DNA sequences within the nucleus. To trace specific DNA sequences in living cells, we used programmable sequence-specific DNA binding of designer transcription activator-like effectors (dTALEs).
Carmen Dominguez-Brauer et al.
Cell stem cell, 19(2), 205-216 (2016-05-18)
The E3 ubiquitin ligase Mule is often overexpressed in human colorectal cancers, but its role in gut tumorigenesis is unknown. Here, we show in vivo that Mule controls murine intestinal stem and progenitor cell proliferation by modulating Wnt signaling via c-Myc.
The linker histone H1. 2 is a novel component of the nucleolar organizer regions
Junjie C, et al.
The Journal of Biological Chemistry (2018)
Ellen Markljung et al.
PLoS biology, 7(12), e1000256-e1000256 (2009-12-18)
A single nucleotide substitution in intron 3 of IGF2 in pigs abrogates a binding site for a repressor and leads to a 3-fold up-regulation of IGF2 in skeletal muscle. The mutation has major effects on muscle growth, size of the
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