B6916
Réactif de Bradford
for 0.1-1.4 mg/ml protein
Synonyme(s) :
Dosage des protéines par liaison du bleu de Coomassie, réactif de coloration des protéines
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About This Item
Nom du produit
Réactif de Bradford, for 0.1-1.4 mg/ml protein
form
solution
storage temp.
2-8°C
Quality Level
Catégories apparentées
Application
Features and Benefits
- Ce réactif est prêt à utiliser. Aucune dilution ou aucun mélange nécessaire.
- La couleur se développe rapidement. Une incubation de cinq minutes suffit avant la lecture de l′échantillon à 595 nm.
- Les sucres réducteurs et les substances réductrices ou les thiols n′interfèrent pas avec ce réactif.
- Ce réactif convient aux essais aux formats micro (1-10 μg/ml) et standard (50-1400 μg/ml).
- Il peut être utilisé dans les essais en microplaques.
- Essai peu coûteux.
General description
Legal Information
signalword
Warning
hcodes
Hazard Classifications
Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2 - STOT SE 2
target_organs
Eyes,Central nervous system
Classe de stockage
8B - Non-combustible corrosive hazardous materials
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
Will the buffer or solution my protein is in interfere with Product B6916, Bradford Reagent?
A compatibility chart is listed in the bulletin. If your substance is not listed, then we recommend testing this by diluting the standard protein samples in the same buffer as the unknown samples.
What is the useful concentration range that can be measured by Product B6916, Bradford Reagent?
The Bradford Reagent requires no dilution and is suitable for micro, multiwell plate, and standard (cuvet) assays. The linear concentration range is 0.1-1.4 mg/mL of protein, using BSA (bovine serum albumin) as the standard protein.
When using Product B6916, Bradford Reagent, how soon after my color development do I need to read my assay?
he protein-dye complex is stable up to 60 minutes. The absorbency of the samples must be recorded before the 60 minute time limit and within 10 minutes of each other.
When using Product B6916, Bradford Reagent, what can I do if I have a very dilute sample in a large volume?
The micro assay using this same reagent may be an option for you. The micro assay is used when a large volume (at least 1 mL) of a dilute sample is available for testing. The linear concentration range of this assay is lower than the standard or multiwell plate assays, (1-10 μg of total protein in 1 mL).
Is the BSA standard supplied with Product B6916, Bradford Reagent?
No, we do not supply the BSA standard with this reagent. We recommend using one of the following products: Protein Standard (BSA) Solution, (2 mg/mL), Product No. P0834 or Protein Standard (BSA) Solution, (1 mg/mL), Product No. P0914, if lower concentrations of protein are to be measured.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I find price and availability?
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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