B7435
CelLytic™ B Cell Lysis Reagent
For bacterial cell lysis, standard strength
Synonyme(s) :
Cell lysis reagent
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Catégories apparentées
Application
A proprietary, non-denaturing formulation of zwitterionic detergents used for the lysis of bacterial cells and extraction of recombinant proteins.
CelLytic™ B Cell Lysis Reagent has been used for cell lysis for the extraction of coenzyme Q10, recombinant Src kinase associated phosphoprotein 1- glutathione S-transferases (GST) fusion protein and human α-synuclein.
Features and Benefits
- Higher protein extraction efficiency than traditional methods such as sonication and lysozyme
- Scalable for 1 to 25 grams of bacterial cell paste
- No interference with downstream applications such as affinity chromatography, IP, and Western blotting
- Compatible with protease inhibitors, inhibitor cocktails, chaotropes, salts, chelating agents and reducing agents
Other Notes
Detergent blend formulated in 40 mM Trizma® HCl (pH 8.0).
Legal Information
Covered by US Patent No 7,282,475 B2 and are sold for research use only. Commercial use requires addtional licenses.
CelLytic is a trademark of Sigma-Aldrich Co. LLC
Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany
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Warning
hcodes
Hazard Classifications
Eye Irrit. 2 - Skin Irrit. 2
Classe de stockage
12 - Non Combustible Liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type ABEK (EN14387) respirator filter
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Ken Motohashi
BMC biotechnology, 15, 47-47 (2015-06-04)
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Arvind R Subramaniam et al.
Proceedings of the National Academy of Sciences of the United States of America, 110(6), 2419-2424 (2013-01-02)
The genetic code underlying protein synthesis is a canonical example of a degenerate biological system. Degeneracies in physical and biological systems can be lifted by external perturbations, thus allowing degenerate systems to exhibit a wide range of behaviors. Here we
Ca2+ increases the specific coenzyme Q10 content in Agrobacterium tumefaciens
Ha SJ, et al.
Bioprocess and Biosystems Engineering, 32(5), 697-700 (2009)
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Raab M, et al.
BMC Research Notes, 11(1), 869-869 (2018)
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