B9673
1-Bromo-3-chloropropane
for isolation of RNA
Synonyme(s) :
Chlorure bromure de triméthylène, I-BCP
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A propos de cet article
Formule linéaire :
Cl(CH2)3Br
Numéro CAS:
Poids moléculaire :
157.44
UNSPSC Code:
41105515
NACRES:
NA.52
PubChem Substance ID:
EC Number:
203-697-1
Beilstein/REAXYS Number:
605278
MDL number:
Assay:
≥98.5% (GC)
Grade:
Molecular Biology
Bp:
144-145 °C (lit.)
Service technique
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Molecular Biology
Quality Level
assay
≥98.5% (GC)
form
liquid
refractive index
n20/D 1.486 (lit.)
bp
144-145 °C (lit.)
density
1.592 g/mL at 25 °C (lit.)
storage temp.
room temp
SMILES string
ClCCCBr
InChI
1S/C3H6BrCl/c4-2-1-3-5/h1-3H2
InChI key
MFESCIUQSIBMSM-UHFFFAOYSA-N
General description
BCP can be used in place of chloroform and is less toxic. The use of BCP in the single-step TRI reagent method decreases the possibility of contaminating RNA with DNA. It does not adversely affect quality or quantity of the isolated RNA. 1-Bromo-3-chloropropane is a phase separation reagent used in protocols for isolation of nucleic acids.
Application
1-Bromo-3-chloropropane has been used in molecular biology applications for the isolation of high quality RNA from:
- feline teeth
- mice brain and liver tissues
- human ectocervical epithelial cells
- placental samples
- human mesenchymal stromal cells (hMSCs)
Features and Benefits
- less toxic alternative to chloroform
- compatible with TRI reagent for isolation of RNA with decreased DNA contamination
Other Notes
BCP can be used in place of chloroform, and is less toxic. The use of BCP in the single-step TRI Reagent method decreases the possibility of contaminating RNA with DNA. It does not adversely affect quality or quantity of the isolated RNA.
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signalword
Danger
hcodes
Hazard Classifications
Acute Tox. 3 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Muta. 2 - STOT SE 3
target_organs
Respiratory system
Classe de stockage
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
wgk
WGK 2
ppe
Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter
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Microarray methods for drug discovery, 27-44 (2010)
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de Carvalho TS, et al.
Translational Stroke Research, 1-12 (2019)
Estradiol alters the immune-responsiveness of cervical epithelial cells stimulated with ligands of Toll-like receptors 2 and 4
Lashkari BS and Anumba DOC
PLoS ONE, 12(3), e0173646-e0173646 (2017)