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Merck

C0775

Violet cristallisé

Dye content, ≥90%, certified by the Biological Stain Commission, powder

Synonyme(s) :

Chlorure d’hexaméthylpararosaniline, Violet 3 de base, Violet de gentiane, Violet de méthyle 10B

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About This Item

Formule linéaire :
C25H30N3Cl
Numéro CAS:
548-62-9
Poids moléculaire :
407.98
UNSPSC Code:
12171500
NACRES:
NA.47
PubChem Substance ID:
57653980
EC Number:
208-953-6
MDL number:
MFCD00011750
Colour Index Number:
42555
Beilstein/REAXYS Number:
3580948

Nom du produit

Violet cristallisé, certified by the BSC

SMILES string

[Cl-].CN(C)c1ccc(cc1)\C(c2ccc(cc2)N(C)C)=C3/C=C\C(C=C3)=[N+](/C)C

InChI key

ZXJXZNDDNMQXFV-UHFFFAOYSA-M

InChI

1S/C25H30N3.ClH/c1-26(2)22-13-7-19(8-14-22)25(20-9-15-23(16-10-20)27(3)4)21-11-17-24(18-12-21)28(5)6;/h7-18H,1-6H3;1H/q+1;/p-1

agency

certified by the BSC

form

powder

composition

Dye content, ≥90%

technique(s)

microbe id | staining: suitable

color

green to very dark green

pH

2.5 — 3.5 (20 °C)

mp

205 °C (dec.) (lit.)

solubility

H2O: 1 mg/mL

density

1.19 g/cm3 at 20 °C

antibiotic activity spectrum

fungi

application(s)

diagnostic assay manufacturing
hematology
histology

mode of action

cell membrane | interferes
enzyme | inhibits

storage temp.

room temp

Quality Level

200

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Catégories apparentées

Application

Certified for use with the Gram stain for bacteria, Flemming triple stain with iodine for chromosomes, Lieb′s amyloid stain and Newton′s crystal violet-iodine technique for chromatin and nucleoli.
Crystal violet is used as an active component, primary stain, of Gram stain for differentiation of Gram-negative versus Gram-positive bacteria.
It has been used to check cell viability. It has also been used for the staining of cells to study cell migration and invasion.

Biochem/physiol Actions

Crystal violet can be used for DNA visualization in agarose gels. The dye is used only in the presence of high concentrations of DNA. Crystal violet is also used for the staining of bacteria in gram staining technique. It is also used for the staining of plant chromosomes. Crystal Violet also helps in colorimetric measurement of cell viability.

signalword

Danger

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 2 - Eye Dam. 1

Classe de stockage

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificats d'analyse (COA)

Lot/Batch Number
0000499856
0000499855
0000499855
0000499856
SHBS9966

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Consulter la Bibliothèque de documents

Lipocalin 2 induces the epithelial-mesenchymal transition in stressed endometrial epithelial cells: possible correlation with endometriosis development in a mouse model.
Liao CJ, et al.
Reproduction (Cambridge, England), 147, 179-179 (2013)
A combined assay of cell viability and in vitro cytotoxicity with a highly water-soluble tetrazolium salt, neutral red and crystal violet.
Ishiyama M, et al.
Biological & Pharmaceutical Bulletin, 19, 1518-1518 (1996)
Vera van de Pol et al.
International journal of molecular sciences, 20(13) (2019-07-05)
Bicuspid aortic valve (BAV), the most common congenital heart defect, is associated with an increased prevalence of aortic dilation, aortic rupture and aortic valve calcification. Endothelial cells (ECs) play a major role in vessel wall integrity. Little is known regarding
Carson S and Robertson D
Manipulation and Expression of Recombinant DNA (2005)
Ki Hyun Kim et al.
PLoS pathogens, 15(2), e1007375-e1007375 (2019-02-02)
Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease localized to China, Japan, and Korea that is characterized by severe hemorrhage and a high fatality rate. Currently, no specific vaccine or treatment has been approved for this disease.
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