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Merck

C4951

Cholesterol-Water Soluble

powder, BioReagent, suitable for cell culture

Synonyme(s) :

Cholesterol–methyl-β-cyclodextrin

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A propos de cet article

NACRES:
NA.75
UNSPSC Code:
12352211
Service technique
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biological source

ovine wool

Quality Level

product line

BioReagent

form

powder

technique(s)

cell culture | mammalian: suitable

solubility

H2O: 300 mg/mL

shipped in

ambient

storage temp.

−20°C

Application

Cholesterol-Water Soluble has been used:
  • in free cholesterol (FC) overloading of rat smooth muscle cells (SMCs)
  • in cytotoxicity assay
  • for the incubation of CD4+ T cells with Helicobacter pylori
  • to study the membrane cholesterol level in SH-SY5Y human neuroblastoma cells
  • to study the effects of cholesterol on cell viability and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reduction in primary cultured rat hippocampal neurons
  • as a supplement for growth of bacterial culture
  • to examine the interaction of S protein with angiotensin-converting enzyme 2 (ACE2)
  • to investigate the effects of cholesterol on potassium currents in the inner hair cells of guinea pigs
  • for reversible inhibition of proteinase K

Packaging

Package size based on cholesterol

Preparation Note

Preparea solution at 300mg/mL in water. Let thesolution sit undisturbed for one hour after diluting to obtain completesolubilization of product.

Other Notes

Contains approx. 40 mg of cholesterol per gram; balance methyl-β-cyclodextrin.


Classe de stockage

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)



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Consulter la Bibliothèque de documents



Importance of cholesterol-rich membrane microdomains in the interaction of the S protein of SARS-coronavirus with the cellular receptor angiotensin-converting enzyme 2
Glende J, et al.
Virology, 381(2), 215-221 (2008)
Cholesterol and its derivatives reversibly inhibit proteinase K
Singh N and Bhattacharyya D
Journal of Cellular Physiology, 232(3), 596-609 (2017)
A novel reciprocal and biphasic relationship between membrane cholesterol and beta-secretase activity in SH-SY5Y cells and in human platelets
Liu WW, et al.
Journal of Neurochemistry, 108(2), 341-349 (2009)