C9080
Monoclonal Anti-Vimentin−Cy3 antibody produced in mouse
clone V9, purified immunoglobulin, buffered aqueous solution
Synonyme(s) :
Monoclonal Anti-Vimentin
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A propos de cet article
NACRES:
NA.41
UNSPSC Code:
12352203
Conjugate:
CY3 conjugate
Clone:
V9, monoclonal
Application:
IF (d), IHC (f), IHC (p)
Citations:
33
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Laissez-nous vous aiderbiological source
mouse
conjugate
CY3 conjugate
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
V9, monoclonal
form
buffered aqueous solution
mol wt
antigen ~58 kDa
species reactivity
pig, canine, feline, hamster, rabbit, gerbil, monkey, bovine, chicken, human, horse, rat
technique(s)
direct immunofluorescence: 1:200 using cultured cells, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50 using human tonsil, immunohistochemistry (frozen sections): suitable using human tonsil
isotype
IgG1
UniProt accession no.
shipped in
wet ice
storage temp.
2-8°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... VIM(7431)
rat ... Vim(81818)
General description
Monoclonal Anti-Vimentin (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from immunized BALB/c mice. Vimentin is expressed in breast myoepithelial cells, osteocytes, Langerhans cells of the skin, Schwann cells and astrocytes.
Vimentin is one of the five major groups of intermediate filaments with a molecular mass of 57 kDa. Intermediate filaments (IFs) with characteristic 10 nm diameter are a distinct class of molecularly heterogenous cytoskeletal filaments defined by ultrastructural, immunological and biochemical criteria. IFs differ significantly from the other cytoskeletal elements of the cell, namely microtubules and microfilaments and are components of most eukaryotic cells.
Immunogen
pig eye lens vimentin.
Application
Monoclonal Anti-Vimentin-Cy3 antibody produced in mouse has been used in:
- immunofluorescence and confocal microscopy
- cytoskeleton staining
- double labeling experiments
- immunohistochemical
- immunocytochemical localozation
Biochem/physiol Actions
Cy3 Monoclonal Anti-Vimentin reacts with normal and pathological tissue of mesenchymally-derived cells, lens tissue and various cultured cells. It localizes vimentin in fibroblasts, lipocytes, endothelial cells, some lymphoid cells, melanocytes, macrophages, and chondrocytes. The antibody stains tumors derived from these cells, including sarcomas, most lymphomas, melanomas and their metastatic lesions. Co-expression of vimentin with keratin was described in most carcinomas of certain sites (e.g., kidney and thyroid) and certain carcinomas of other sites (e.g., breast).
The antibody localizes vimentin in fibroblasts, endothelial cells, lymphoid tissue and melanocytes in immunohistochemical staining. It also stains vimentin in sarcomas, lymphomas, and melanomas.
Vimentin was found to be important for stabilizing the architecture of the cytoplasm. It was found, that activated macrophages secrete vimentin into the extracellular space in vitro.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide
Preparation Note
Store at 2-8 °C. Protect from prolonged exposure to light. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
Legal Information
Cy is distributed under license from Amersham Biosciences Limited.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Classe de stockage
10 - Combustible liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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Coexpression of keratin-and vimentin-type intermediate filaments in human metastatic carcinoma cells
Ramaekers FC, et al.
Proceedings of the National Academy of Sciences of the USA, 80(9), 2618-2622 (1983)
Guangheng Li et al.
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, 20(9), 1611-1623 (2005-08-02)
After intramuscular implantation, BMP4-expressing NIH/3T3 fibroblasts and BMP4-expressing C2C12 myoblasts can promote ectopic cartilage and bone formation. Fibroblasts tend to undergo chondrogenesis, whereas myoblasts primarily undergo osteogenesis. These results suggest that endochondral bone formation may involve different cell types, a
Yimu Zhao et al.
Matrix biology : journal of the International Society for Matrix Biology, 85-86, 189-204 (2019-04-15)
Organ-on-a-chip systems have the potential to revolutionize drug screening and disease modeling through the use of human stem cell-derived cardiomyocytes. The predictive power of these tissue models critically depends on the functional assembly and maturation of human cells that are
Sven Otto et al.
PloS one, 16(5), e0251530-e0251530 (2021-05-21)
Distal axonopathy is seen in a broad range of species including equine patients. In horses, this degenerative disorder of the recurrent laryngeal nerve is described as recurrent laryngeal neuropathy (RLN). The dysfunctional innervation of the cricoarytenoideus dorsalis muscle (CAD) leads
Morphology of the myoepithelial cell: immunohistochemical characterization from resting to motile phase
Beha G, et al.
TheScientificWorldJournal, 2012 (2012)
Contenu apparenté
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