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Merck

NIH 3T3 Cell Line murine

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 93061524, mouse embryo, Fibroblast

Synonyme(s) :

3T3 Cells, Mouse 3T3 Cells, NIH/3T3 Cells, NIH3T3 Cells, Swiss 3T3 Cells, Swiss3T3 Cells

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A propos de cet article

UNSPSC Code:
41106514
Biological source:
mouse embryo
Growth mode:
Adherent
Karyotype:
Not specified
Morphology:
Fibroblast

Principaux documents

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biological source

mouse embryo

description

Embryonic fibroblast

packaging

tube of 5 μg 93061524-DNA-5UG, pkg of vial of cells 93061524-1VL

growth mode

Adherent

karyotype

Not specified

morphology

Fibroblast

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

shipped in

dry ice

storage temp.

−196°C

Catégories apparentées

Application

DNA transfection studies
NIH 3T3 Cell Line has been used to:
  • study calcium-mediated actin reset (CaAR) in response to physiological changes
  • study the effects of activation of the transient receptor potential vanilloid 3 channel (TRPV3) on adipocyte differentiation
  • determine the viral titer of the retroviral construct -ST40hTNFα

Biochem/physiol Actions

Established from a NIH Swiss mouse embryo. These cells are sensitive to sarcoma virus focus formation and leukemia virus propogation. Cells have now lost their contact inhibition.
Mouse Swiss NIH embryo

Preparation Note

DMEM + 2mM Glutamine + 10% Calf Serum (CS).
Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-5x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C. Do not allow culture to become fully confluent; the use of fetal calf serum is not recommended.

Other Notes

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