CS0170
Thioredoxin Reductase Assay Kit
1 kit sufficient for 100 assays (1 mL)
Synonyme(s) :
Thioredoxin Reductase Activity Kit
Se connecter pour consulter les tarifs organisationnels et contractuels.
Sélectionner une taille de conditionnement
A propos de cet article
NACRES:
NA.84
UNSPSC Code:
12161503
Service technique
Besoin d'aide ? Notre équipe de scientifiques expérimentés est là pour vous.
Laissez-nous vous aiderService technique
Besoin d'aide ? Notre équipe de scientifiques expérimentés est là pour vous.
Laissez-nous vous aiderusage
kit sufficient for 100 assays (1 mL)
shipped in
dry ice
storage temp.
−20°C
Quality Level
Gene Information
human ... TXNRD1(7296), TXNRD2(10587), TXNRD3(114112)
Catégories apparentées
General description
The Thioredoxin Reductase Assay Kit uses a colorimetric assay for the determination of thioredoxin reductase activity in mammalian cells and tissues. It is based on the reduction of 5,5′-dithiobis(2-nitrobenzoic) acid (DTNB) with NADPH to 5-thio-2-nitrobenzoic acid (TNB) that produces a strong yellow color that is measured at 412 nm.
DTNB + NADPH + H+ ↔ 2 TNB + NADP+
DTNB + NADPH + H+ ↔ 2 TNB + NADP+
Application
Using post nuclear extracts of rat liver, the kit shows linearity up to 0.25 units per mL. In this preparation, ~20% of the activity is due to non-specific reductase activity (refractory to the TrxR inhibitor).
Features and Benefits
- An easy and simple method
- The kit contains all the reagents required for a colorimetric assay of mammalian thioredoxin reductase
- The kit includes an inhibitor solution for specific inhibition of mammalian thioredoxin reductase
- The kit has been tested with heart muscle, as well as lysates from cell lines such as HeLa, A549, Jurkat, U937, A431, COS, CHO, and NIH 3T3 cells
Composants de kit seuls
Réf. du produit
Description
- Assay Buffer 5x for Thioredoxin Reductase 30 mL
- Thioredoxin Reductase 10 μg
- Thioredoxin Reductase Inhibitor Solution .05 mL
- 5,5'-Dithiobis(2-nitrobenzoic) acid (DNTB) 150 mg
- NADPH 25 mg
- Dimethyl Sulfoxide (DMSO) 7.5 mL
signalword
Warning
Classe de stockage
10 - Combustible liquids
flash_point_f
188.6 °F
flash_point_c
87 °C
hcodes
Hazard Classifications
Eye Irrit. 2 - Repr. 2 - STOT SE 2
target_organs
Eyes,Central nervous system
wgk
WGK 3
Faites votre choix parmi les versions les plus récentes :
Déjà en possession de ce produit ?
Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Matthew T Harper
Platelets, 30(1), 98-104 (2017-12-02)
Platelets are central to normal hemostasis and must be tightly controlled to prevent thrombosis. However, drug treatments that also affect platelets could lead to unwanted side effects on hemostasis or thrombosis. In this study, the effect of auranofin on platelets
Tessa D Epstein et al.
ACS infectious diseases, 5(10), 1682-1687 (2019-09-06)
Helicobacter pylori (H. pylori) infection poses a worldwide public health crisis, as chronic infection is rampant and can lead to gastric ulcers, gastritis, and gastric cancer. Unfortunately, frontline therapies cause harmful side effects and are often ineffective due to antibiotic resistance.
Ida Landini et al.
Journal of inorganic biochemistry, 208, 111079-111079 (2020-06-05)
The antiproliferative properties of a series of structurally-related gold(I) and silver(I) linear complexes inspired to the clinically established gold-based drug auranofin were investigated in A2780 ovarian cancer cells and in their auranofin (A2780/AF-R) and cisplatin (A2780/CDDP-R) resistant counterparts. In A2780
Iwona Jarocka-Karpowicz et al.
Biomolecules, 10(3) (2020-03-04)
Psoriasis is a chronic inflammatory skin disease characterized by dysregulated keratinocyte differentiation, but oxidative stress also plays an important role in the pathogenesis of this disease. Here, we examined the effect of cannabidiol (CBD), a phytocannabinoid with antioxidant and anti-inflammatory
Melissa A Fath et al.
Clinical cancer research : an official journal of the American Association for Cancer Research, 17(19), 6206-6217 (2011-08-17)
Cancer cells (relative to normal cells) show increased steady-state levels of hydroperoxides that are compensated by increased glucose and hydroperoxide metabolism. The current study determined whether inhibitors of glucose and hydroperoxide metabolism could induce chemoradiosensitization by enhancing oxidative stress in
Contenu apparenté
Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..
Contacter notre Service technique