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Merck

CS1000

Superoxide Anion Assay Kit

sufficient for 100 assays (96 well plates)

Synonyme(s) :

Superoxide Detection Kit

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A propos de cet article

NACRES:
NA.84
UNSPSC Code:
12161503

Principaux documents

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usage

sufficient for 100 assays (96 well plates)

shipped in

wet ice

storage temp.

2-8°C

Quality Level

200

Catégories apparentées

Application

The Superoxide Anion assay kit provides a useful qualitative cell-based assay for the measurement of O2− anion status in cells. The kit can be used to test changes in superoxide anion levels following oxidative stress directly in whole cells. In addition, the kit can be also used for the detection of superoxide dismutase (SOD) activity. This activity was tested on erythrocytes, leucocytes, and plasma.

Biochem/physiol Actions

The superoxide anion (O2-) is a short-lived radical generated by the addition of an electron to oxygen. It is formed in response to environmental factors such as UV light, cigarette smoke, environmental pollutants, and γ-radiation, or by oxidases like xanthine oxidase or NADPH oxidase. Once formed, O2- attacks cellular components causing damage to lipids, proteins, and DNA. This can initiate numerous diseases, including cancer, atherosclerosis, rheumatoid arthritis, diabetes, liver damage, and central nervous system disorders.

Analysis Note

The kit method is based on the oxidation of luminol by superoxide anions resulting in the formation of chemiluminescence light. This method utilizes a specific, non-toxic enhancer that amplifies the chemiluminescent signal.

Composants de kit seuls

Réf. du produit
Description
  • Assay Buffer 25 mL
  • Assay Medium 20 mL
  • Enhancer Solution, 40 mM 500 μL
  • Luminol Solution 500 μL
  • Xanthine Solution 500 μL
  • Xanthine Oxidase .5 U
  • Superoxide Dismutase from bovine liver, 4 units/μL 400 U

Classe de stockage

10 - Combustible liquids

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Certificats d'analyse (COA)

Lot/Batch Number
0000160563
0000160563
0000114035
089M4044V
11190616

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Consulter la Bibliothèque de documents

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Macrophages serve as permissive niches for Escherichia coli (E. coli) K1 to attain high grade bacteremia in the pathogenesis of meningitis in neonates. Although pterin levels are a diagnostic marker for immune activation, the role of macrophages in pterin production
Hyunsoo Chung et al.
Frontiers in cell and developmental biology, 10, 800574-800574 (2022-06-17)
Podocyte loss is well known to play a critical role in the early progression of diabetic nephropathy. A growing number of studies are paying attention to necroptosis, a programmed form of cell necrosis as a mechanism of podocyte loss. Although
Dawei Jiang et al.
Nature biomedical engineering, 2(11), 865-877 (2018-12-07)
Patients with acute kidney injury (AKI) frequently require kidney transplantation and supportive therapies, such as rehydration and dialysis. Here, we show that radiolabelled DNA origami nanostructures (DONs) with rectangular, triangular and tubular shapes accumulate preferentially in the kidneys of healthy
Erythropoietin activates nitric oxide synthase in murine erythrocytes
Mihov,D et al.
The American Journal of Physiology, 279(2), C378-C388 (2009)
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