D2944
Anti-D1 Dopamine Receptor antibody,Rat monoclonal
clone 1-1-F11 s.E6, purified from hybridoma cell culture
Synonyme(s) :
Anti-DRD1
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A propos de cet article
Conjugate:
unconjugated
Clone:
1-1-F11 s.E6, monoclonal
Application:
ICC, IHC, WB
Citations:
60
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Laissez-nous vous aiderbiological source
rat
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
1-1-F11 s.E6, monoclonal
form
buffered aqueous solution
mol wt
antigen ~50 kDa
species reactivity
rat, monkey, human
concentration
~1 mg/mL
technique(s)
immunocytochemistry: 5-10 μg/mL using D1 dopamine receptor transfected HEK-293T cells, immunohistochemistry: suitable, western blot: suitable
isotype
IgG2a
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... DRD1(1812)
rat ... Drd1a(24316)
General description
D1 Dopamine Receptor encodes the D1 subtype of the dopamine receptor. The D1 subtype is the most abundant dopamine receptor in the central nervous system. This G-protein coupled receptor stimulates adenylyl cyclase and activates cyclic AMP-dependent protein kinases.
Monoclonal Anti-D1 Dopamine Receptor (rat IgG2a isotype) is derived from the rat hybridoma 1-1-F11 S.E6 produced by the fusion of mouse myeloma cells and splenocytes from rat immunized with recombinant fusion protein containing the C-terminal 97 amino acid of human D1 dopamine receptor. Dopamine receptors belong to the family of seven transmembrane domain G protein coupled receptors (GPCRs). Dopamine receptors are mainly localized in the striatum, limbic system, the brain cortex and the infundibulum. The D1-like family consists of the D1 and D5 receptors.
Immunogen
recombinant fusion protein containing the C-terminal 97 amino acid of human D1 dopamine receptor.
Application
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)
Immunohistochemistry (1 paper)
Immunofluorescence (1 paper)
Immunohistochemistry (1 paper)
Monoclonal Anti-D1 Dopamine Receptor antibody produced in rat has been used in:
- immunohistochemistry
- immunoblotting
- immunocytochemistry
- proximity ligation assay
- co-immunoprecipitation
- western blotting
Biochem/physiol Actions
D1 receptors regulate neuronal growth and development, mediate some behavioral responses, and modulate dopamine receptor D2-mediated events. D1 receptors are expressed on airway smooth muscle (ASM) and regulate smooth muscle force via cAMP activation of protein kinase A. It is a potential target for therapeutic relaxation of ASM.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Legal Information
Sold under exclusive license from Emory University
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Classe de stockage
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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Kentaro Mizuta et al.
Respiratory research, 14, 89-89 (2013-09-06)
Dopamine signaling is mediated by Gs protein-coupled "D1-like" receptors (D1 and D5) and Gi-coupled "D2-like" receptors (D2-4). In asthmatic patients, inhaled dopamine induces bronchodilation. Although the Gi-coupled dopamine D2 receptor is expressed and sensitizes adenylyl cyclase activity in airway smooth
Eleanor H Simpson et al.
Biological psychiatry, 76(10), 823-831 (2014-01-07)
Evidence indicating an increase in dopamine D2 receptor (D2R) density and occupancy in patients with schizophrenia comes from positron emission tomography studies using ligands that bind both D2Rs and dopamine D3 receptors (D3Rs), questioning the role of D3Rs in the
Satoshi Goto et al.
Frontiers in neuroanatomy, 9, 22-22 (2015-03-19)
Immunohistochemistry (IHC) is a valuable method for identifying discrete neurochemical molecules by the interaction of target antigens with validated antibodies tagged with a visible label (e.g., peroxidase). We have developed an immunostaining method that is highly sensitive in detection of
D1R/GluN1 complexes in the striatum integrate dopamine and glutamate signalling to control synaptic plasticity and cocaine-induced responses
Cahill E, et al.
Molecular Psychiatry, 19(12), 1295-1295 (2014)
Impact of early consumption of high-fat diet on the mesolimbic dopaminergic system
Naneix F, et al.
eNeuro, 4(3) (2017)
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