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Merck

E1388

β-Endothelial Cell Growth Factor human

≥97% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

Synonyme(s) :

ECGS, β-ECGF

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A propos de cet article

Numéro CAS:
105843-51-4
UNSPSC Code:
12352202
NACRES:
NA.77
MDL number:
MFCD00131012
Biological source:
human
Recombinant:
expressed in E. coli
Assay:
≥97% (SDS-PAGE)
Form:
lyophilized powder
Mol wt:
predicted mol wt ~17 kDa
Impurities:
≤1.0 EU/μg protein Endotoxin level (LAL test)

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Nom du produit

β-Endothelial Cell Growth Factor human, β-ECGF, recombinant, expressed in E. coli, suitable for cell culture

biological source

human

recombinant

expressed in E. coli

assay

≥97% (SDS-PAGE)

form

lyophilized powder

potency

0.03-0.3 ng/mL ED50/EC50

quality

endotoxin tested

mol wt

predicted mol wt ~17 kDa

packaging

pkg of 25 μg

storage condition

avoid repeated freeze/thaw cycles (Do not store in a frost-free freezer.)

technique(s)

cell culture | mammalian: suitable

impurities

≤1.0 EU/μg protein Endotoxin level (LAL test)

UniProt accession no.

P49765

storage temp.

−20°C

Quality Level

200

Gene Information

human ... VEGFB(7423)

Catégories apparentées

General description

The β-endothelial cell growth factor (b-ECGF) gene encodes an angiogenic factor, and is mapped to human chromosome 11q13.1. Recombinant, human b-endothelial cell growth factor (b-ECGF) is a 14 amino acid N-terminal extension of acidic fibroblast growth factor. It is expressed in Escherichia coli. The 154 amino acid recombinant protein, generated after removal of the initiation methionine, has a predicted molecular mass of about 17 kDa.

Application

β-Endothelial Cell Growth Factor human has been used:
  • in cell death assay
  • in endothelial cell culture
  • in human umbilical vascular endothelial cell (HUVEC) culture

Biochem/physiol Actions

β-Endothelial cell growth factor (β-ECGF) is a potent endothelial cell mitogen and its mitogenic activity is potentiated by heparin. β-ECGF stimulates the growth of quiescent HUVEC cells in culture. β-ECGF is associated with lipid and glucose metabolism. This growth factor induces neovascularization in disease conditions. Upregulation of β-ECGF is observed in diabetic retinopathy, and polycystic ovary syndrome. It is also associated with tumor proliferation and invasion. β-ECGF increases fatty acid transport proteins expression to improve endothelial mediated lipid transpor.

Physical form

Lyophilized from a 0.2 μm filtered solution in 10 mM MOPS, 50 mM ammonium sulfate, 0.2 mM DTT, and 0.2 mM EDTA, pH 7.2, containing 50 μg bovine serum albumin per 1 μg cytokine.

Analysis Note

The proliferative activity is tested in culture by using quiescent NR6R-3T3 fibroblasts.

Other Notes

14 amino acid, N-terminal extended form of human FGF-acidic.

Classe de stockage

11 - Combustible Solids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificats d'analyse (COA)

Lot/Batch Number
0000513044
0000513044
0000511509
0000511509
0000506971

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Consulter la Bibliothèque de documents

Autologous cell lines from circulating colon cancer cells captured from sequential liquid biopsies as model to study therapy-driven tumor changes
Soler A, et al.
Scientific reports, 8(1), 15931-15931 (2018)
Plasma vascular endothelial growth factor B levels are increased in patients with newly diagnosed type 2 diabetes mellitus and associated with the first phase of glucose-stimulated insulin secretion function of beta-cell
Wu J, et al.
Journal of Endocrinological Investigation, 40(11), 1219-1226 (2017)
Vascular endothelial growth factor B and its signaling
Lal N, et al.
Frontiers in cardiovascular medicine, 5(18), 39-39 (2018)
Bioinformatics analyses of the role of vascular endothelial growth factor in patients with non-small cell lung cancer
Wang Y, et al.
PLoS ONE, 10(9), e0139285-e0139285 (2015)
A blood pressure-associated variant of the SLC39A8 gene influences cellular cadmium accumulation and toxicity
Zhang R, et al.
Human Molecular Genetics, 25(18), 4117-4126 (2016)
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Contenu apparenté

Data Sheet - E1388

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