F1392
Riboflavin 5′-monophosphate sodium salt hydrate
suitable for electrophoresis, suitable for acrylamide photopolymerization, ≥70%, powder
Synonyme(s) :
FMN-Na, Flavin mononucleotide, Riboflavin 5′-phosphate sodium salt
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A propos de cet article
Formule empirique (notation de Hill) :
C17H20N4NaO9P · xH2O
Numéro CAS:
Poids moléculaire :
478.33 (anhydrous basis)
UNSPSC Code:
41106305
NACRES:
NA.51
PubChem Substance ID:
EC Number:
204-988-6
Beilstein/REAXYS Number:
4106529
MDL number:
Assay:
≥70%
Form:
powder
Solubility:
H2O: soluble 50 mg/mL, clear, orange
Storage temp.:
−20°C
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BHRVCJBIICJWTH-APQIITSESA-M
InChI
1S/C17H21N4O9P.Na.H2O/c1-7-3-9-10(4-8(7)2)21(15-13(18-9)16(25)20-17(26)19-15)5-11(22)14(24)12(23)6-30-31(27,28)29;;/h3-4,11-12,14,22-24H,5-6H2,1-2H3,(H,20,25,26)(H2,27,28,29);;1H2/q;+1;/p-1/t11-,12+,14-;;/m0../s1
SMILES string
O.[Na+].Cc1cc2N=C3C(=O)NC(=O)N=C3N(C[C@H](O)[C@H](O)[C@H](O)COP(O)([O-])=O)c2cc1C
assay
≥70%
form
powder
optical activity
[α]20/D +37 to +42°, c = 1.5 in 5 M HCl(lit.)
technique(s)
electrophoresis: suitable
solubility
H2O: soluble 50 mg/mL, clear, orange
ε (extinction coefficient)
12.5 at 445 nm at 1 mM
suitability
suitable for acrylamide photopolymerization
foreign activity
Protease, none detected
storage temp.
−20°C
Quality Level
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Catégories apparentées
Application
Riboflavin 5′-monophosphate sodium salt hydrate or FMN is suitable:
- to study its mechanism of inhibition of mutagenicity of a benzo[a]pyrene 7,8-diol 9,10-epoxide, a carcinogenic metabolite of benzo[a]pyrene
- along with flavin adenine dinucleotide for reactivation of the enzyme that catalyzes the oxidation of pyridoxamine to pyridoxal in a study
- to study the reduction of polymeric azo and nitro dyes by intestinal bacteria
- to study the effect of FMN on ferrisiderophore reductase activity in the cytoplasmic fraction of Agrobacterium tumefaciens.
Biochem/physiol Actions
FMN is a coenzyme synthesized from riboflavin (vitamin B2). It acts as a component of complex I of the electron transport chain. The enzymes containing FMN are called as flavin enzymes and are involved in oxidation-reduction mechanisms.
FMN is suitable as a photopolymerization reagent in polyacrylamide gel electrophoresis (PAGE) by forming free radicals in aqueous solution in the presence of light. FMN photodecomposes to leucoflavin. No free radicals are formed in the absence of oxygen, but traces of oxygen allow for leucoflavin to reoxidize with free-radical generation. The catalysts, tetramethylethylenediamine (TEMED) or 3-dimethylaminopropionitrile (DMAPN), are commonly added to speed up the free radical formation. Free radicals will cause acrylamide and bis-acrylamide to polymerize to form a gel matrix which can be used for sieving macromolecules. FMN is commonly used in the stacking gel for non-denaturing PAGE because native proteins can be sensitive to persulfate ions from ammonium persulfate. Another advantage of FMN over ammonium persulfate is that it will not start polymerizing until the gel is illuminated.
FMN is suitable as a photopolymerization reagent in polyacrylamide gel electrophoresis (PAGE) by forming free radicals in aqueous solution in the presence of light. FMN photodecomposes to leucoflavin. No free radicals are formed in the absence of oxygen, but traces of oxygen allow for leucoflavin to reoxidize with free-radical generation. The catalysts, tetramethylethylenediamine (TEMED) or 3-dimethylaminopropionitrile (DMAPN), are commonly added to speed up the free radical formation. Free radicals will cause acrylamide and bis-acrylamide to polymerize to form a gel matrix which can be used for sieving macromolecules. FMN is commonly used in the stacking gel for non-denaturing PAGE because native proteins can be sensitive to persulfate ions from ammonium persulfate. Another advantage of FMN over ammonium persulfate is that it will not start polymerizing until the gel is illuminated.
Riboflavin 5′-monophosphate or Flavin mononucleotide (FMN) serves as a prosthetic group for flavoenzymes. It is a part of the mitochondrial respiratory chain complex I and acts as a redox carrier. FMN is unstable in alkaline solution and is highly stable at pH 6.
Classe de stockage
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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J P Brown
Applied and environmental microbiology, 41(5), 1283-1286 (1981-05-01)
The O(2)-sensitive reduction of high-molecular-weight aromatic azo and nitro dyes by intestinal bacteria appears to be mediated by low-molecular-weight electron carriers with E(o)' = -200 to -350 mV. This process may allow the design of polymeric azo prodrugs for specific
Matthias Mack et al.
Applied microbiology and biotechnology, 71(3), 265-275 (2006-04-12)
Flavins are active components of many enzymes. In most cases, riboflavin (vitamin B(2)) as a coenzyme represents the catalytic part of the holoenzyme. Riboflavin is an amphiphatic molecule and allows a large variety of different interactions with the enzyme itself
A W Wood et al.
Proceedings of the National Academy of Sciences of the United States of America, 79(17), 5122-5126 (1982-09-01)
Riboflavin 5'-phosphate (flavin mononucleotide; FMN) inhibits the mutagenicity of (+/-)-7 beta, 8 alpha-dihydroxy-9 alpha, 10 alpha-epoxy-7,8,9,10-tetrahydrobenzo[a]pyrene (B[a]P diol epoxide), the only known ultimate carcinogenic metabolite of benzo[a]pyrene. Coincubation of 10, 25, and 50 nmol of FMN with strain TA100 of
J S Lodge et al.
Journal of bacteriology, 149(2), 771-774 (1982-02-01)
Reduction of the iron in ferriagrobactin by the cytoplasmic fraction of Agrobacterium tumefaciens strictly required NaDH as the reductant. Addition of flavin mononucleotide and anaerobic conditions were necessary for the reaction; when added with flavin mononucleotide, magnesium was stimulatory. This
P G Righetti et al.
Electrophoresis, 14(7), 573-582 (1993-07-01)
The kinetics of monomer incorporation into a polyacrylamide gel have been studied in a photopolymerization system comprising 100 microM methylene blue in presence of a red-ox system, 1 mM sodium toluenesulfinate (reducer) and 50 microM diphenyliodonium chloride (oxidizer). A precise
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