Accéder au contenu
Merck

F2426

Gel d′affinité ANTI-FLAG® M2 EZview™ Rouge

clone M2

Synonyme(s) :

Anticorps monoclonal ANTI-FLAG® M2 antibody produced in mouse, Anti-ddddk, Anti-dykddddk

Se connecter pour consulter les tarifs organisationnels et contractuels.

Sélectionner une taille de conditionnement

Toutes les photos(1)

About This Item

NACRES:
NA.32
UNSPSC Code:
12352203

Nom du produit

Gel d′affinité ANTI-FLAG® M2 EZview™ Rouge, clone M2

Quality Level

200

clone

M2, monoclonal

analyte chemical class(es)

proteins

technique(s)

affinity chromatography: suitable
immunoprecipitation (IP): suitable

matrix

4% agarose bead; 45-165μm bead size

isotype

IgG1

capacity

≥0.6 mg/mL, gel binding capacity

shipped in

wet ice

storage temp.

−20°C

Vous recherchez des produits similaires ? Visite Guide de comparaison des produits

Catégories apparentées

Application

Immunoprécipitation (IP) des protéines de fusion portant une étiquette FLAG ou 3×FLAG.

Élution - peptide FLAG, glycine, pH 3,5, peptide 3xFLAG.

Pour en savoir plus sur nos produits, rendez-vous sur notre portail des applications des produits FLAG®.

Biochem/physiol Actions

Ce produit peut être utilisé pour la purification de protéines de fusion portant des étiquettes FLAG N-terminale, Met-N-terminale et C-terminale ainsi que de protéines de fusion portant une étiquette 3×FLAG.

Disclaimer

Gels d'affinité FLAG®, étiquette FLAG®, étiquette 3xFLAG®, étiquette DYKDDDDK

General description

Le gel d'affinité anti-FLAG M2 EZview Rouge est une résine comprenant des anticorps anti-FLAG M2 immobilisés par liaison covalente sur des billes d'agarose à 4 %. Ce gel d'affinité permet de lier des protéines de fusion portant une étiquette FLAG à des échantillons tels que des tissus ou des lysats cellulaires dans le but de purifier les protéines à étiquette FLAG lors de la préparation d'analyses par immunoprécipitation. Le colorant rouge améliore la visibilité, et donc, la performance des résultats. Les billes d'agarose lient les protéines de fusion portant des étiquettes FLAG N-terminale, Met-N-terminale et C-terminale ainsi que les protéines de fusion portant une étiquette 3×FLAG.

Physical form

Suspension à 1:1 (v/v) dans du PBS contenant 50 % de glycérol et 15 ppm de Kathon.

Legal Information

ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
EZview is a trademark of Sigma-Aldrich Co. LLC
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

Classe de stockage

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Faites votre choix parmi les versions les plus récentes :

Certificats d'analyse (COA)

Lot/Batch Number
0000441904
0000441904
0000432486
0000432486
0000269913

Vous ne trouvez pas la bonne version ?

Si vous avez besoin d'une version particulière, vous pouvez rechercher un certificat spécifique par le numéro de lot.

Rechercher un(e) COA

Déjà en possession de ce produit ?

Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. When using Product F2426, EZview™ Red ANTI-FLAG® M2 Affinity Gel clone M2, should I use a 3X FLAG peptide or a 1X FLAG peptide to elute my protein?

    If you have a 3X FLAG-tagged protein, then you will need to use the 3X FLAG peptide.  If you have a 1X FLAG-tagged protein, you can use the 1X FLAG peptide or the 3X FLAG peptide.  We have not noticed a significant  difference in elution efficiency by using a 3X FLAG peptide on a 1X FLAG-tagged protein.

  3. When using Product F2426, EZview™ Red ANTI-FLAG® M2 Affinity Gel clone M2, how can I elute my protein?

    Elution with the peptide is the most gentle method.  Acid elution (0.1 M glycine-HCL pH 3.5) is a more stringent method of elution, and should be evaluated for its effect on your protein if it is to be used in downstream applications.  Boiling the resin in sample buffer is the most denaturing condition.  If this condition is used, the resin cannot be re-used, due to the presence of SDS and/or reducing agents.

  4. When using Product F2426, EZview™ Red ANTI-FLAG® M2 Affinity Gel clone M2, I see bands at 20-25 kDa and 50-60 kDa appearing in my Westerns that are not my FLAG-tagged protein. How can I prevent this?

    As a result of the conjugation, there may be some M2 antibody that is not conjugated to the resin, but is associated with the resin and may appear in acid elutions as heavy and light chain when using the anti-mouse IgG conjugated secondary antibody.  We recommend a acid wash (0.1 M glycine-HCL pH 3.5) and neutralization of the resin (do not allow the acid wash to sit on the resin longer than 20 minutes) prior to applying the lysate.  Another way to avoid this is to use a directly conjugated FLAG antibody for detection such as product A8592 ant-FLAG M2 HRP, or the rabbit anti-FLAG polyclonal antibody, F7425.

  5. When using Product F2426, EZview™ Red ANTI-FLAG® M2 Affinity Gel clone M2, I have a lot of non-specific proteins that are eluting with my FLAG-tagged protein. How can I get rid of these?

    One way to remove non-specific proteins is to pre-bind the protein lysate with unconjugated resin.  We recommend product 4B200 for this purpose. Other methods would be to increase the stringency of the washes by increasing salt concentration (the resin can tolerate up to 1M NaCl) or including detergents that are compatible with the resin.

  6. What is the binding capacity of the Product F2426, EZview™ Red ANTI-FLAG® M2 Affinity Gel clone M2, resin?

    The binding capacity of the resin must be   ? 0.6 mg/mL to meet specifications.  This capacity will vary from lot to lot. 

  7. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  8. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  9. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  10. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Kara R Jones et al.
Molecular cancer therapeutics, 4(10), 1541-1547 (2005-10-18)
Incomplete DNA repair or misrepair can contribute to the cytotoxicity of DNA double-strand breaks. Consequently, interference with double-strand break repair, by pharmacologic or genetic means, is likely to sensitize tumor cells to ionizing radiation. The current studies were designed to
Matthew S Walters et al.
Journal of virology, 84(13), 6861-6865 (2010-04-16)
Varicella zoster virus encodes an immediate-early (IE) protein termed ORF61p that is orthologous to the herpes simplex virus IE protein ICP0. Although these proteins share several functional properties, ORF61p does not fully substitute for ICP0. The greatest region of similarity
Jiahai Zhou et al.
Proceedings of the National Academy of Sciences of the United States of America, 103(39), 14343-14348 (2006-09-16)
The unfolded protein response (UPR) is an evolutionarily conserved mechanism by which all eukaryotic cells adapt to the accumulation of unfolded proteins in the endoplasmic reticulum (ER). Inositol-requiring kinase 1 (IRE1) and PKR-related ER kinase (PERK) are two type I
Matthew Frieman et al.
Journal of virology, 83(13), 6689-6705 (2009-04-17)
The outcome of a viral infection is regulated in part by the complex coordination of viral and host interactions that compete for the control and optimization of virus replication. Severe acute respiratory syndrome coronavirus (SARS-CoV) intimately engages and regulates the
Ting-Ting Qu et al.
BMC cancer, 18(1), 27-27 (2018-01-06)
Lin28B and its paralog Lin28A are small RNA binding proteins that have similar inhibitory effects, although they target separate steps in the maturation of let-7 miRNAs in mammalian cells. Because Lin28B participates in the promotion and development of tumors mostly
Afficher tous les articles scientifiques apparentés

Contenu apparenté

eView Resins
EZview Red Protein A and ANTI-FLAG M2 Affinity Gels

EZviewTM Red Protein A and ANTI-FLAG® M2 Affinity Gels: Immunoprecipitation with Enhanced Visibility Affinity Beads - Technical Article - July 2001

Technical Bulletin - F2426

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

Contacter notre Service technique