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Merck

F5636

Monoclonal Anti-FITC antibody produced in mouse

clone FL-D6, ascites fluid

Synonyme(s) :

Monoclonal Anti-FITC

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A propos de cet article

NACRES:
NA.46
UNSPSC Code:
12352203
Conjugate:
unconjugated
Clone:
FL-D6, monoclonal
Application:
indirect ELISA
indirect immunofluorescence
Technique(s):
indirect ELISA: 1:2,500 using FITC-conjugate
indirect immunofluorescence: suitable
Citations:
7
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Nom du produit

Monoclonal Anti-FITC antibody produced in mouse, clone FL-D6, ascites fluid

biological source

mouse

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

FL-D6, monoclonal

contains

15 mM sodium azide

technique(s)

indirect ELISA: 1:2,500 using FITC-conjugate
indirect immunofluorescence: suitable

isotype

IgG1

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

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Application

Mouse monoclonal clone FL-D6 anti-FITC antibody may be used for the detection of FITC and as a universal indicator reagent for bridging FITC with other immunochemical reagents. It may be used in ELISA, competitive ELISA and immunofluorescent techniques. A FITC Anti-FITC system has been used in the amplification of signal in immunofluorescent detection and as a means of separating bound from free tracer by affinity chromatography. The antibody can also be used to isolate cells that have an FITC-labeled ligand on their surface.

Biochem/physiol Actions

Mouse monoclonal clone FL-D6 anti-FITC antibody will react with either free or conjugated FITC. The antibody does not react with bound or free TRITC (tetramethylrhodamine isothiocyanate).
The antibody reacts with both free and conjugated fluorescein isothiocyanate (FITC).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

FITC (fluorescein isothiocyanate) is a fluorochrome dye that absorbs ultraviolet or blue light causing molecules to become excited and emit a visible yellow-green light. This emission ceases upon removal of the light causing the excitation. Fluorochrome labeling provides rapid, accurate localization of antigen-antibody interaction when one of the reactants is part of a cell, tissue or other biological structure. FITC is a commonly used marker for antibodies in immunofluorescent techniques because the conjugation of FITC to proteins is relatively easy and generally does not destroy the biological activity of the labeled protein. FITC is widely used as a hapten to label different proteins. Antibodies to FITC are used to identify FITC labeled proteins and as models to study the mechanism of antibody response to a well defined hapten.

Immunogen

FITC-BSA conjugate

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Classe de stockage

10 - Combustible liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable


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Sandrine Tury et al.
Oncotarget, 7(51), 85124-85141 (2016-11-12)
COX-2 expression level and prognostic value are still a matter of debate in breast cancer (BC). We addressed these points in the context of PIK3CA mutational status. Based on an interesting study of aspirin efficacy in colorectal cancer, we hypothesized
Hiam Abdala-Valencia et al.
Journal of immunology (Baltimore, Md. : 1950), 177(9), 6379-6387 (2006-10-24)
Lymphocyte binding to VCAM-1 activates endothelial cell NADPH oxidase, resulting in the generation of 1 muM H(2)O(2). This is required for VCAM-1-dependent lymphocyte migration. In this study, we identified a role for protein kinase Calpha (PKCalpha) in VCAM-1 signal transduction
R Chignola et al.
Clinical and experimental immunology, 122(3), 429-436 (2000-12-21)
Two monoclonal antibodies (1H6.2 and 45.30) were raised against MBP purified from human brain under experimental conditions that allowed MBP to retain binding to surrounding myelin lipids (human lipid-bound MBP (hLB-MBP)). 1H6.2 and 45.30 MoAbs were selected on the basis
Dongxi Xiang et al.
Theranostics, 5(10), 1083-1097 (2015-07-23)
Insufficient penetration of therapeutic agents into tumor tissues results in inadequate drug distribution and lower intracellular concentration of drugs, leading to the increase of drug resistance and resultant failure of cancer treatment. Targeted drug delivery to solid tumors followed by
Pedro M Costa et al.
Molecular therapy. Nucleic acids, 2, e100-e100 (2013-06-20)
The present work aimed at the development and application of a lipid-based nanocarrier for targeted delivery of nucleic acids to glioblastoma (GBM). For this purpose, chlorotoxin (CTX), a peptide reported to bind selectively to glioma cells while showing no affinity

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