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A propos de cet article
Numéro CAS:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-864-1
MDL number:
Specific activity:
≥500 units/mg protein
Recombinant:
expressed in E. coli
Service technique
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Laissez-nous vous aiderrecombinant
expressed in E. coli
Quality Level
form
lyophilized powder
specific activity
≥500 units/mg protein
mol wt
465 kDa
storage temp.
−20°C
General description
Poids moléculaire du tétramère : 465 kDa (chaque sous-unité ayant 116,3 kDa)
β-galactosidase is a 465 kDa tetrameric protein. It is coded by the LacZ gene.
Application
β-Galactosidase may be used for derivatization, such as an enzyme label for IgG, without prior dialysis or gel filtration.
β-Galactosidase from Escherichia coli has been used to produce biocatalytic Ca-alginate beads on a B-390 encapsulator (BUCHI). It has been used to prepare the source droplet solution and also as the cargo for Pep-1 (KETWWETWWTEWSQPKKKRKV-cysteamide).
Biochem/physiol Actions
βLa β-galactosidase clive le lactose en ses constituants monosaccharides, le glucose et le galactose. Elle catalyse également la transglycosylation du glucose en allolactose, inducteur de la β-galactosidase, dans une boucle de rétroaction.
β-galactosidase is considered as a reporter enzyme in applications involving gene expression regulation, analysis of protein function/structure and target gene expression.
Packaging
Package size based on protein content
Physical form
Stabilized with phosphate buffer and sucrose
Other Notes
One unit will hydrolyze 1.0 μmole of o-nitrophenyl β-D-galactopyranoside to o-nitrophenol and D-galactose per min at pH 7.3 at 37 °C.
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Classe de stockage
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Contenu apparenté
Datasheet
Peptide-Mediated Membrane Transport of Macromolecular Cargo Driven by Membrane Asymmetry
Li X, et al.
Analytical Chemistry, 89(22), 12369-12374 (2017)
M J O'Sullivan et al.
Annals of clinical biochemistry, 16(5), 221-240 (1979-09-01)
In the last few years, the use of enzyme labels in immunoassays has been investigated. The aim of this review is to evaluate critically the role of such labels in clinical biochemistry. Special attention has been given to the problems
Structure of beta-galactosidase at 3.2-AA resolution obtained by cryo-electron microscopy
Bartesaghi A, et al.
Proceedings of the National Academy of Sciences of the USA, 111(32), 11709-11709 (2014)