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Merck

G5160

β-Galactosidase from Aspergillus oryzae

≥8.0 units/mg solid

Synonyme(s) :

β-D-Galactoside galactohydrolase, Lactase from Aspergillus oryzae

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A propos de cet article

Numéro CAS:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-864-1
MDL number:
Numéro CE :
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Nom du produit

β-Galactosidase from Aspergillus oryzae, ≥8.0 units/mg solid

form

solid

specific activity

≥8.0 units/mg solid

contains

dextrin as extender

storage temp.

−20°C

Quality Level

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Application

β-Galactosidase from Aspergillus oryzae has been used:
  • in in vitro nuclear magnet resonance (NMR)
  • as a standard in the β-galactosidase assay to determine the concentration of cell-associated β-galactosidase
  • to modify sugar residues on the surface of the mutant slime cells to study the adhesive properties
  • for enzymatic hydrolysis of lactose
  • for reversed-phase (RP) adsorption
  • in the hydrolysis of whey lactose

Biochem/physiol Actions

β-Galactosidase acts on the terminal β-D-galactosyl moieties of disaccharides, glycoconjugates, and polysaccharides. It is useful in mediating transglycosylation reactions such as the synthesis of galactooligosaccharides (GOS). β-Galactosidases might be used for glycobiological and biotechnological applications.
β-galactosidase cleaves lactose into its monosaccharide components, glucose and galactose. It also catalyses the transglycosylation of glucose into allolactose, the inducer of β-galactosidase, in a feedback loop.

General description

β-Galactosidase is a member of the glycoside hydrolase clan A (GH-A) family. It is widely present in microorganisms, which includes both prokaryotes and eukaryotes.

Other Notes

One unit will hydrolyze 1.0 μmole of lactose per minute at pH at 4.5 at 30 °C.

Classe de stockage

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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Applied microbiology and biotechnology, 98(8), 3553-3567 (2013-09-17)
Galactosidases are widespread enzymes that are used for manifold applications, including production of prebiotics, biosynthesis of different transgalactosylated products, improving lactose tolerance and in various analytical approaches. The nature of these applications often require galactosidases to be present in a

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