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Merck

G6532

Anti-GroEL antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonyme(s) :

Anti-GroEL, GroEL Antibody - Anti-GroEL antibody produced in rabbit - Tested Applications: immunoblotting 1:80,000,indirect ELISA 1:40,000-1:80,000 - Sigma Aldrich G6532, Groel Antibody

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A propos de cet article

NACRES:
NA.46
UNSPSC Code:
12352203
Conjugate:
unconjugated
Clone:
polyclonal
Application:
ELISA (i), WB
Citations:
61

Principaux documents

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biological source

rabbit

conjugate

unconjugated

antibody form

IgG fraction of antiserum

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

E. coli

technique(s)

indirect ELISA: 1:40,000-1:80,000, western blot: 1:80,000

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

200

Gene Information

Escherichia coli ... groEL(913705), groEL(913705)
Escherichia coli K12 ... Grol(948665)

General description

GroEL protein belongs to the folding chaperonin family. It is a 58.3 kDa protein with apical, intermediate and equatorial functional domains.

Immunogen

purified recombinant GroEL produced in E. coli.

Application

Anti-GroEL antibody produced in rabbit has been used as positive control in histology and immunohistochemistry of Dirofilaria immitis, nematode worms and in the western blot analysis of the HeLa cells.

Biochem/physiol Actions

GroEL, together with GroES, in the presence of ATP, assists in protein folding. Co-expression of aldehyde dehydrogenase with GroEL/GroES promotes folding and higher production of protein in soluble form. GroES and GroEL bind to the protein substrate, enclosing it in a cage like fold. The target protein after folding gets dissociated from the cage and is accompanied with hydrolysis of adenosine triphosphate (ATP) to adenosine diphosphate (ADP).
The antibody does not cross-react with GroES and bovine HSP 70.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Classe de stockage

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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Certificats d'analyse (COA)

Lot/Batch Number
0000447549
0000447549
0000342519
0000342519
0000213581

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Consulter la Bibliothèque de documents

Functional characterization of an archaeal GroEL/GroES chaperonin system Significance of substrate encapsulation.
Figueiredo L, et al.
The Journal of Biological Chemistry, 279(2), 1090-1099 (2004)
The chlamydial periplasmic stress response serine protease cHtrA is secreted into host cell cytosol.
Wu X, et al.
BMC Microbiology, 11(1), 87-87 (2011)
Crystal Structure of Escherichia coli GroEL in Substrate and ADP Unloaded State.
Meena S and Saxena AK
Journal of Physical Chemistry & Biophysics, 6(222), 2161-0398 (2016)
Daniela Dimastrogiovanni et al.
eLife, 3 (2015-01-01)
Bacterial small RNAs (sRNAs) are key elements of regulatory networks that modulate gene expression. The sRNA RydC of Salmonella sp. and Escherichia coli is an example of this class of riboregulators. Like many other sRNAs, RydC bears a 'seed' region
Chaperonin GroESL mediates the protein folding of human liver mitochondrial aldehyde dehydrogenase in Escherichia coli.
Lee KH, et al.
Biochemical and Biophysical Research Communications, 298(2), 216-224 (2002)
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Contenu apparenté

Data Sheet - G6532

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