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Merck

GAGO20

Kit de dosage du glucose (GO)

sufficient for 20 assays

Synonyme(s) :

Kit de dosage du glucose

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A propos de cet article

NACRES:
NA.84
UNSPSC Code:
12164500

Principaux documents

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Nom du produit

Kit de dosage du glucose (GO), sufficient for 20 assays

usage

sufficient for 20 assays

Quality Level

100

species reactivity

human

packaging

pkg of 1 kit

storage condition

dry at room temperature

technique(s)

activity assay: suitable

input

food(s)

accession no.

P35557
NM_000162.5

application(s)

food and beverages
general analytical

detection method

colorimetric

storage temp.

2-8°C

Catégories apparentées

Application

Adapté à la détermination enzymatique quantitative du glucosedans les aliments et autres produits.
Ce kit est destiné à la détermination enzymatique quantitative du glucose dans les aliments et autres produits. Le glucose est oxydé en acide gluconique et en peroxyde d'hydrogène par la glucose oxydase. Le peroxyde d'hydrogène réagit avec l'o-dianisidine en présence de peroxydase pour former un produit coloré. L'o-dianisidine oxydée réagit avec l'acide sulfurique pour former un produit coloré plus stable. L'intensité de la coloration rose mesurée à 540 nm est proportionnelle à la concentration d'origine en glucose.

Composants de kit seuls

Réf. du produit
Description
  • o-dianisidine reagent 1 mL/vial
  • glucose oxidase/peroxidase reagent 1 capsule
  • glucose standard .5 mL

signalword

Danger

Hazard Classifications

Acute Tox. 4 Oral - Carc. 1B - Eye Dam. 1 - Skin Corr. 1

Classe de stockage

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk

WGK 3

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Certificats d'analyse (COA)

Lot/Batch Number
0000518206
0000518206
0000502684
0000502684
0000495175

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

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  1. Can Product GAGO20, Glucose (GO) Assay Kit, be tested on blood or plasma glucose?

    For analysis of such samples, this kit should be useful on samples that have been deproteinized. 

  2. Can Product GAGO20, Glucose (GO) Assay Kit, be used in a 96-well plate format?

    Sigma Aldrich has not performed this in a 96-well plate format.  However if a method has have not tried this. If you have developed a method in a 96-well format, Sigma Aldrich would be happy to know about this.

  3. Can frozen samples be tested with Product GAGO20, Glucose (GO) Assay Kit?

    One publication has successfully reported use of the GAGO20 on samples frozen at -20 °C.

  4. Can Product GAGO20, Glucose (GO) Assay Kit, be used on culture media samples?

    Sigma Aldrich has not tested this product on culture media samples, but several publications in the literature report successful use of the kit with culture media samples.

  5. What is the difference between the GAGO20 and GAHK20 Glucose (GO) Assay Kits?

    The kits use different enzymes. GAGO20 uses glucose oxidase and peroxidase, while GAHK20 uses hexokinase.  Furthermore, the final reaction products are monitored at different wavelengths, 540 nm (GAGO20) and 340 nm (GAHK20).

  6. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  7. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  8. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  9. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  10. How would I decolorize my sample for use in the glucose assay using Product GAGO20, Glucose (GO) Assay Kit?

    First, you will need to determine if the color affects the result by reading it as a blank in the assay. The classical method for decolorization of carbohydrate solutions is treatment with charcoal. A small amount of powdered charcoal such as Product C3345 is added to the sample, stirred a few moments, then clarified by filtration or centrifugation. If the color persists, another portion of charcoal is added and the process repeated until there is no more change in color with an addition of charcoal.

  11. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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Natural plant pigment, anthocyanins have the capability to change its color with the change of its structure influenced by changing pH. This feature of anthocyanin has been harnessed to design a meat products quality indicator. In the present experiment anthocyanin
Bergmeyer, H.U. and Bernt, E.,
Methods of Enzymatic Analysis, 1205-1212 (1974)
Nelson H Knudsen et al.
Science (New York, N.Y.), 368(6490) (2020-05-02)
Repeated bouts of exercise condition muscle mitochondria to meet increased energy demand-an adaptive response associated with improved metabolic fitness. We found that the type 2 cytokine interleukin-13 (IL-13) is induced in exercising muscle, where it orchestrates metabolic reprogramming that preserves
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Disordered folliculogenesis is a core characteristic of polycystic ovary syndrome (PCOS) and androgen receptors (ARs) are closely associated with hyperandrogenism and abnormalities in folliculogenesis in PCOS. However, whether the new AR binding partner phosphoglycerate kinase 1 (PGK1) in granulosa cells
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