Accéder au contenu
Merck

H1404

Hepatocyte Growth Factor human

>95% (SDS-PAGE), recombinant, expressed in Baculovirus infected High-5 cells, lyophilized powder, suitable for cell culture

Synonyme(s) :

HGF, Hepatopoietin A, Scatter Factor

Se connecter pour consulter les tarifs organisationnels et contractuels.

Sélectionner une taille de conditionnement

A propos de cet article

UNSPSC Code:
12352202
NACRES:
NA.77
EC Number:
214-275-1
MDL number:
MFCD00217422
Biological source:
human
Recombinant:
expressed in Baculovirus infected High-5 cells
Assay:
>95% (SDS-PAGE)
Form:
lyophilized powder
Mol wt:
~80 kDa
Impurities:
<1.0 EU/μg

Principaux documents

Voir toute la documentation
Service technique
Besoin d'aide ? Notre équipe de scientifiques expérimentés est là pour vous.
Laissez-nous vous aider
Service technique
Besoin d'aide ? Notre équipe de scientifiques expérimentés est là pour vous.
Laissez-nous vous aider

Nom du produit

Hepatocyte Growth Factor human, HGF, recombinant, expressed in Baculovirus infected High-5 cells, suitable for cell culture

biological source

human

recombinant

expressed in Baculovirus infected High-5 cells

assay

>95% (SDS-PAGE)

form

lyophilized powder

potency

1.0-20.0 ng/mL

quality

endotoxin tested

mol wt

~80 kDa

packaging

pkg of 5x5 μg, pkg of 5 μg

storage condition

avoid repeated freeze/thaw cycles

technique(s)

cell culture | mammalian: suitable

impurities

<1.0 EU/μg

UniProt accession no.

P14210

storage temp.

−20°C

Quality Level

200

Gene Information

human ... HGF(3082)

Vous recherchez des produits similaires ? Visite Guide de comparaison des produits

Catégories apparentées

Biochem/physiol Actions

Hepatocyte growth factor (HGF) is a potent mitogen for hepatocytes and a variety of cell types including endothelial and epithelial cells, melanocytes, and keratinocytes. It also mediates epithelial morphogenesis. HGF is the ligand for the tyrosine kinase receptor encoded by the met proto-oncogene.

Physical form

Lyophilized from a 0.2 μm filtered solution in 20 mM NaH2PO4 and 500 mM NaCl, pH 7.0 with 50 μg BSA per 1 μg HGF

Preparation Note

Reconstitute at 50 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. After reconstitution, this solution can be stored at 2-8 °C for up to one month or at -20 °C to -70 °C for up to three months. Us a manual defrost freezer and avoid repeated freeze-thaw cycles.

Analysis Note

The biological activity is measured by its ability to induce IL-11 secretion by Saos-2 human osieosarcoma cells.

Other Notes

The NSO-expressed recombinant HGF is greater than 95% heterodimeric HGF. The insect cell line-expressed recombinant HGF is a mixture of single chain HGF (predominant) with some heterodimeric HGF. Single chain HGF and heterodimeric HGF are equally active in in vitro assays.

Classe de stockage

11 - Combustible Solids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

Faites votre choix parmi les versions les plus récentes :

Certificats d'analyse (COA)

Lot/Batch Number
0000252232
0000252227
0000252232
SLCP0076
SLCP0078

Vous ne trouvez pas la bonne version ?

Si vous avez besoin d'une version particulière, vous pouvez rechercher un certificat spécifique par le numéro de lot.

Rechercher un(e) COA

Déjà en possession de ce produit ?

Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Masaki Kobayashi et al.
Biochemical and biophysical research communications, 527(1), 153-161 (2020-05-25)
MicroRNA (miRNA) plays a pivotal role in cancer biology. Therefore, tumor suppressor (TS) miRNAs are an attractive target for cancer therapy. However, clinical trials have failed due to the difficulties in miRNA delivery, warranting the development of a novel drug
Tumay Basar et al.
Infection and immunity, 73(4), 2061-2074 (2005-03-24)
The bacterial pathogen Listeria monocytogenes causes food-borne illnesses leading to gastroenteritis, meningitis, or abortion. Listeria induces its internalization into some mammalian cells through interaction of the bacterial surface protein InlB with host Met receptor tyrosine kinase. Binding of InlB leads
Takanori Takebe et al.
Nature protocols, 9(2), 396-409 (2014-01-25)
Generation of functional and vascularized organs from human induced pluripotent stem cells (iPSCs) will facilitate our understanding of human developmental biology and disease modeling, hopefully offering a drug-screening platform and providing novel therapies against end-stage organ failure. Here we describe
R A Furlong
BioEssays : news and reviews in molecular, cellular and developmental biology, 14(9), 613-617 (1992-09-01)
Hepatocyte growth factor, a potent mitogen for epithelial and other cell types, and scatter factor, a stimulant of epithelial cell motility are identical. In addition to these mitogenic and motogenic functions, the factor has been shown to be an epithelial
Yingjie Liu et al.
Development (Cambridge, England), 136(5), 843-853 (2009-01-30)
Integrin receptors for the extracellular matrix and receptor tyrosine kinase growth factor receptors represent two of the major families of receptors that transduce into cells information about the surrounding environment. Wnt proteins are a major family of signaling molecules that
Afficher tous les articles scientifiques apparentés

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

Contacter notre Service technique