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Merck

H2387

Hanks′ Balanced Salts

Modified, without calcium chloride, magnesium sulfate and sodium bicarbonate, powder, suitable for cell culture

Synonyme(s) :

HBSS

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A propos de cet article

UNSPSC Code:
12352207
NACRES:
NA.75
MDL number:
Service technique
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form

powder

technique(s)

cell culture | mammalian: suitable

storage temp.

2-8°C

General description

Balanced salt solution (BSS) provides cells with water, bulk inorganic ions, and buffering system to maintain the normal cell metabolism and physiological range (pH 7.2-7.6), respectively. It also acts as an irrigating, diluting, and transporting agent and maintains the intracellular and extracellular osmotic balance. BSS also provides energy sources such as glucose for cell metabolism.

Application

Hanks′ Balanced Salt has been used:

  • to culture primary hippocampal neuron cells from rats
  • to collect the rat brains
  • to culture rat primary hepatocytes

Preparation Note

Formulated to contain 9.5 grams of powder per liter of medium.
Supplement with 0.35 g/L sodium bicarbonate.

Other Notes

The pH is adjusted after addition of sodium bicarbonate.


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Classe de stockage

13 - Non Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)



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Bikendra Maharjan et al.
Materials science & engineering. C, Materials for biological applications, 114, 111056-111056 (2020-10-01)
Despite intensive attempts to fabricate polypyrrole nanoparticles (PPy-NPs) incorporated nanofibrous scaffolds, a low-cost facile strategy is still demanded. Herein, we developed a novel strategy- in-situ polymerization of PPy-NPs and immobilized them into the PCL polymeric matrix in a single step.
Seo Yeon Lee et al.
Polymers, 12(2) (2020-02-20)
Herein, we describe precisely a covalent modification of pure magnesium (Mg) surface and its application to induce in vitro osteogenic differentiation. The new concept of a chemical bonding method is proposed for developing stable chemical bonds on the Mg surface
Abdelrahman I Rezk et al.
Scientific reports, 9(1), 117-117 (2019-01-16)
Magnesium (Mg) and its alloys have attracted much attention as a promising candidate for degradable implant applications however the rapid corrosion of magnesium inside the human body greatly limits its use as an implant material. Therefore, coating the alloy surface