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Merck

H3784

HEPES sodium salt

BioPerformance Certified, suitable for cell culture, ≥99.0%

Synonyme(s) :

Acide 4-(2-hydroxyéthyl)pipérazine-1-éthanesulfonique sodium salt, Acide N-(2-hydroxyéthyl)pipérazine-N′-(2-éthanesulfonique) sodium salt

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A propos de cet article

Formule empirique (notation de Hill) :
C8H17N2NaO4S
Numéro CAS:
75277-39-3
Poids moléculaire :
260.29
NACRES:
NA.25
PubChem Substance ID:
24895595
UNSPSC Code:
12161700
EC Number:
278-169-7
MDL number:
MFCD00036463

Principaux documents

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Nom du produit

HEPES sodium salt, BioPerformance Certified, suitable for cell culture, ≥99.0%

InChI key

RDZTWEVXRGYCFV-UHFFFAOYSA-M

InChI

1S/C8H18N2O4S.Na/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14;/h11H,1-8H2,(H,12,13,14);/q;+1/p-1

SMILES string

[Na+].OCCN1CCN(CC1)CCS([O-])(=O)=O

grade

BioPerformance Certified

assay

≥99.0%

form

powder

storage condition

dry at room temperature

technique(s)

activity assay: suitable
cell culture | mammalian: suitable

impurities

endotoxin and total aerobic microbial count, tested

color

white

pH

10-12 (1 M in water)

useful pH range

6.8-8.2

pKa (25 °C)

7.5

solubility

water: 0.1 M, clear, colorless

density

1.19 g/cm3 at 20—25 °C
1.21 g/cm3 at 20—25 °C

absorption

≤0.02 at 280 in H2O at 0.1 M
≤0.04 at 260 in H2O at 0.1 M

suitability

suitable for component for culture media
suitable for molecular biology

application(s)

cell analysis
diagnostic assay manufacturing
general analytical
life science and biopharma

foreign activity

DNase, RNase, NICKase, protease, none detected

Quality Level

400

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Catégories apparentées

Application

HEPES sodium salt has been used:
  • to supplement the HSOF medium
  • as a buffer component of HCZB medium in egg and embryo culture
  • as a buffer component in cell culture

Features and Benefits

  • High-purity product for biochemical and biological research
  • Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications
  • Highly soluble in water with a useful pH range of 6.8 - 8.2 and pKa of 7.5 at 25 °C
  • Suitable for Cell culture
  • Tested for Endotoxins and Total Aerobic Microbial Count
  • Free from DNase, NICKase, RNase, Endonuclease, Exonuclease and Protease

General description

HEPES, or 4-(2-hydroxyethyl)piperazine-1-ethane-sulfonic acid, is a zwitterionic N-substituted aminosulfonic acid buffer with a remarkable pH buffering capacity between 6.8 and 8.2 (pKa of 7.48 at 25°C). Widely recognized for its versatility, HEPES is extensively employed in cell biology, biochemical, and biological research. Notably, its neutrality towards metal ions makes it a preferred choice for solutions with metal ions, acting as a "non-coordinating buffer."

In cell culture, HEPES excels in maintaining stable physiological pH even amidst fluctuating carbon dioxide concentrations, outperforming bicarbonate buffers (NaHCO3). Moreover, HEPES showcases valuable properties in various biological and biochemical processes, such as isoelectric focusing for protein separation and minimal interference in DNA-restriction enzyme reactions, making it indispensable across diverse research applications, including immunoprecipitation, cell lysis, and live cell imaging. Its versatility, exceptional buffering capacity, and minimal interaction with molecules establish HEPES as a key tool in various research domains.

Other Notes

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Physical form

Lyophilized, spray dried, or crystallized material.

Classe de stockage

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificats d'analyse (COA)

Lot/Batch Number
0000509339
0000509339
0000435518
0000435516
0000435518

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Consulter la Bibliothèque de documents

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Contenu apparenté

Product Information Sheet - H3784

Product Information Sheet

HEPES Sodium Salt Specification Comparison

HEPES Sodium Salt Specification Comparison

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