H8416
Stem Cell Factor human
≥95% (SDS-PAGE), recombinant, expressed in HEK 293 cells, lyophilized powder, suitable for cell culture
Synonyme(s) :
SCF, c-Kit ligand
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A propos de cet article
Biological source:
human
Recombinant:
expressed in HEK 293 cells
Assay:
≥95% (SDS-PAGE)
Form:
lyophilized powder
Mol wt:
dimer 35-45 kDa (glycosylated)
Impurities:
≤1 EU/μg
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Laissez-nous vous aiderNom du produit
Stem Cell Factor human, SCF, recombinant, expressed in HEK 293 cells, suitable for cell culture
biological source
human
recombinant
expressed in HEK 293 cells
assay
≥95% (SDS-PAGE)
form
lyophilized powder
potency
≤200 ng/mL ED50
quality
endotoxin tested
mol wt
dimer 35-45 kDa (glycosylated)
packaging
pkg of 5x10 μg, pkg of 10 μg, pkg of 100 μg
storage condition
avoid repeated freeze/thaw cycles
technique(s)
cell culture | mammalian: suitable
impurities
≤1 EU/μg
UniProt accession no.
storage temp.
−20°C
Quality Level
Gene Information
human ... KITLG(4254)
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General description
The KITLG (KIT ligand) gene is mapped to human chromosome 12q21.32.
Biochem/physiol Actions
Stem Cell Factor (SCF), also known as c-Kit ligand (KL), steel factor (SLF) and mast cell growth factor (MGF), is a 30 kDa glycoprotein with broad activities on various tissues, including hematopoietic cells, pigment cells, and primordial germ cells.
Stem Cell Factor (SCF), also known as c-Kit ligand (KL), steel factor (SLF) and mast cell growth factor (MGF), is a 30 kDa glycoprotein with broad activities on various tissues, including hematopoietic cells, pigment cells, and primordial germ cells. SCF is secreted by endothelial cells, fibroblasts, and bone marrow stromal cells as a membrane-bound form which may be cleaved to release the soluble form. Both forms are active in promoting colony formation from murine bone marrow cells, but membrane-bound SCF is more effective in promoting hematopoieses in vivo, suggesting a role in cellular interactions between hematopoietic and stromal cells. The soluble is thought to exist in solution as a noncovalently linked dimer. SCF is structurally related to M-CSF (CSF-1) and Flt-3/Flk-2 Ligand (FL) with all three sharing a similar size, existence of transmembrane and soluble forms, four conserved cysteines, and alternative splicing exon locations, but they share little sequence homology. SCF alone is a modest colony stimulating factor. However, in the presence of other cytokines such as EPO, TPO, GM-CSF, G-CSF, M-CSF, IL-3, and IL-7, SCF is a potent costimulant that works synergistically to increase the size of myeloid, erythroid or lymphoid lineage colonies without influencing the lineage differentiation of the progenitors.
Stem cell factor (SCF) of granulosa cell origin, efficiently contributes to the follicular development and oocyte maturation. SCF controls tumor development and progression. Elevated SCF gene expression is observed in hepatocellular carcinoma.
Preparation Note
SCF is expressed in human HEK 293 cells as a glycosylated monomer with an apparent molecular mass of 35-45 kDa due to glycosylation. Production in human 293 cells offers authentic glycosylation. Glycosylation contributes to stability in cell growth media and other applications.
Analysis Note
The specific activity was determined by the dose-dependent stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line).
Legal Information
HumanKine is a registered trademark of Proteintech Group, Inc. and Humanzyme, Inc
Classe de stockage
11 - Combustible Solids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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Gene mapping study for constitutive skin color in an isolated Mongolian population
Paik SH, et al.
Experimental & Molecular Medicine, 44, 241-249 (2012)
HIF-2-dependent expression of stem cell factor promotes metastasis in hepatocellular carcinoma
Wang X, et al.
Cancer Letters, 393, 113-124 (2017)
Increased SCF in Follicular Fluid and Granulosa Cells Positively Correlates With Oocyte Maturation, Fertilization, and Embryo Quality in Humans.
Tan J, et al.
Reproductive Sciences, 24(11), 1544-1550 (2017)
Mireille-Maria Suttle et al.
Acta dermato-venereologica, 95(5), 536-541 (2014-11-27)
A slight epidermal damage can induce the Köbner reaction in psoriasis, and the “alarmin”, interleukin-33 (IL-33), may be involved in this process. Therefore, the uninvolved psoriatic skin was tape-stripped, and skin biopsies were collected at 0 day, 2 h and
Yoshimichi Okayama et al.
Immunologic research, 34(2), 97-115 (2006-06-09)
Mast cells play a pivotal role in immediate hypersensitivity and chronic allergic reactions that can contribute to asthma, atopic dermatitis, and other allergic diseases. Because mast cell numbers are increased at sites of inflammation in allergic diseases, pharmacologic intervention into
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