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A propos de cet article
Conjugate:
TRITC conjugate
Clone:
HA-7, monoclonal
Application:
IF (d)
Citations:
9
Service technique
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Laissez-nous vous aiderbiological source
mouse
Quality Level
conjugate
TRITC conjugate
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
HA-7, monoclonal
form
buffered aqueous solution
storage condition
protect from light
concentration
~1 mg/mL
technique(s)
direct immunofluorescence: 10-15 mg/mL using mammalian cells expressing HA-tagged fusion proteins
isotype
IgG1
shipped in
dry ice
storage temp.
−20°C
General description
Monoclonal Anti-HA is derived from the HA-7 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. Monoclonal anti-HA recognizes the HA tag sequence on HA tagged fusion proteins by immunocytochemistry. The antibody reacts specifically with N- and C-terminal HA tagged fusion proteins.
Immunogen
Peptide synthétique correspondant à un fragment de l′hémagglutinine (HA) du virus de la grippe humain, connu sous le nom d′étiquette HA ou HA-tag, conjugué à KLH.
Application
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)
Immunofluorescence (1 paper)
Monoclonal Anti-HA-TRITC antibody was used to test if DOT/ICM effector SDHA is necessary for virulence of Legionella pneumophila in Galleria mellonella and A/J mice.
Biochem/physiol Actions
L′anticorps reconnaît tant les formes natives que dénaturées et réduites des protéines de fusion avec une étiquette HA. Il réagit avec les protéines de fusion exprimées dans E. coli ou dans les cellules de mammifères et dont l′étiquette HA se situe à l′extrémité N-terminale ou C-terminale.
Physical form
Solution in 0.01 M phosphate buffered saline pH 7.4, containing 1% bovine serum albumin 15 mM sodium azide as a preservative.
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Classe de stockage
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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Yasuhiro Yamazaki et al.
Science immunology, 5(44) (2020-03-01)
We investigated the molecular and cellular basis of severe combined immunodeficiency (SCID) in six patients with otofaciocervical syndrome type 2 who failed to attain T cell reconstitution after allogeneic hematopoietic stem cell transplantation, despite successful engraftment in three of them.
D J Scott et al.
British journal of urology, 58(2), 119-124 (1986-04-01)
To assess the effectiveness of the surgical correction of vesico-ureteric reflux, current renal function was determined in 56 children with scarred kidneys who had undergone ureteric reimplantation between 1978 and 1983. The children were aged between 2 and 15 years
P O Olins et al.
Current opinion in biotechnology, 4(5), 520-525 (1993-10-01)
Recent advances in protein expression in E. coli have focused primarily on the enhancement of protein quality. Problems in mRNA translation such as inefficient initiation, mistranslation, frame-shifting and frame-hopping can often be addressed by altering heterologous gene-coding sequences. Fusion technology